fluorescence quenching


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fluor·es·cence quench·ing

a technique used in investigations dealing with binding of antigens (haptens) by purified antibodies, applicable in cases in which the bound antigen (hapten) absorbs (quenches) light emitted during fluorescence of protein (antibody) excited by ultraviolet light.

fluorescence quenching

A technique for investigating antigen-antibody reactions by measuring the light absorbed by an antigen mixed with a fluorescent-labeled antibody.
See also: quenching
References in periodicals archive ?
Fluoranthene tracer was selected to investigate the fluorescence quenching in solution and in TSE because the UV light source for the fluorescence measurement system installed at the die has the highest radiation intensity at 365 nm at which fluoranthene is excited and emits the strongest fluorescence emission.
Discrimination of the photochemical and non-photochemical components of Chl fluorescence quenching is critical to a full understanding of PSII functioning under continuous light, as we will see below.
5) Reduction of fluorescence was caused by increased hydrophobicity of the coating, which facilitated oxygen diffusion into the layer and enhanced oxygen-based fluorescence quenching of the complex.
We monitored the change in fluorescence intensity as oxygen was allowed to diffuse into the film and the presence of oxygen was detected through measurements of fluorescence quenching.
The team named their new technique fluorescence quenching microscopy (FQM).
For field screening at the parts-per-million level, room temperature fluorescence appears satisfactory, since fluorescence quenching by the internal heavy atom effect is not complete.
The mechanism of the fluorescence quenching process was then carefully analyzed using Stern-Volmer equation: [I.
For fluorescence quenching experiments, Stern-Volmer's equation was used (Eq.
Homogeneous time-resolved fluorescence quenching assay (TruPoint) for nucleic acid detection.
6b) when compared with the nonirradiated sample, and this effect probably arises from a diminishing collision-induced fluorescence quenching (30).
As lifetime change is an indicator of fluorescence quenching, the longer lifetime of lanthanide chelates indicated less fluorescence quenching with the surface-coated nanoparticles.
Fluorescence quenching of polyaromatic molecules by electron deficient molecules was extensively studied in liquid solutions and both dynamic (collisional) and static fluorescence quenching mechanisms were developed [1-3].
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