fluorescence quenching


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fluor·es·cence quench·ing

a technique used in investigations dealing with binding of antigens (haptens) by purified antibodies, applicable in cases in which the bound antigen (hapten) absorbs (quenches) light emitted during fluorescence of protein (antibody) excited by ultraviolet light.

fluorescence quenching

A technique for investigating antigen-antibody reactions by measuring the light absorbed by an antigen mixed with a fluorescent-labeled antibody.
See also: quenching
References in periodicals archive ?
The fluorescence quenching of Cry1Ab toxin was measured for a toxin concentration of 40 ug/mL at pH 7, and the concentrations of metal ions changed from 0.
However, the analysis of protein-ligand binding, using fluorescence quenching, may be affected by a number of pitfalls that cannot be easily overcome.
Fluoranthene tracer was selected to investigate the fluorescence quenching in solution and in TSE because the UV light source for the fluorescence measurement system installed at the die has the highest radiation intensity at 365 nm at which fluoranthene is excited and emits the strongest fluorescence emission.
Discrimination of the photochemical and non-photochemical components of Chl fluorescence quenching is critical to a full understanding of PSII functioning under continuous light, as we will see below.
5) Reduction of fluorescence was caused by increased hydrophobicity of the coating, which facilitated oxygen diffusion into the layer and enhanced oxygen-based fluorescence quenching of the complex.
The simplicity of real-time PCR technologies using a fluorescence quenching schemes, allowed diagnosis of the three genotypes of the PSS, and this methodology is a tool that may contribute to the genetic selection and to obtain pigs resistant to PSS.
For the concentration curves of fluorescence quenching by carotenoids, solutions of various carotenoids were spotted onto milled, untreated white rice kernels.
7] M) and showed that 1:1 dye-ion stoichiometry results in nearly complete fluorescence quenching.
More recently, Michael Sailor has demonstrated that a similar fluorescence quenching response can be obtained when porous silicon is exposed to nitroaromatic compounds such as TNT and DNT (2).
Thereafter, steps in the polyphasic transient became less a pparent, with increase in fluorescence quenching with the progress of time (Fig.
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