fluorescence polarization immunoassay


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Related to fluorescence polarization immunoassay: Cloned Enzyme Donor Immunoassay

fluorescence polarization immunoassay

, fluorescent polarization immunoassay Abbreviation: FPIA
A means of identifying and quantifying the amount of an antigen in a specimen in which a fluorescently labeled antibody is mixed with a sample thought to contain the antigen is exposed to polarized light. Bound fluorescently labeled antibody reacts to polarized light in a characteristic fashion not demonstrated by unbound antibody, allowing for identification and measurement of the ligand.
See also: immunoassay
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Proficiency-testing data from the College of American Pathologists for 2010 reveal that 92% of laboratories perform the fluorescence polarization immunoassay used in this case, and all of the 407 participating laboratories currently use an immunoassay platform for MTX.
Aliquots of drug-free serum pools were supplemented with ginseng and apparent digoxin concentrations were measured using enzyme-linked chemiluminescent immunosorbent digoxin assay, turbidimetric assay, and fluorescence polarization immunoassay digoxin assays.
98) 2 Assay Lower limit of Upper limit of method agreement, % agreement, % HPLC(a) -40 15 HPLC(b) -4 62 FPIA -19 16 (a) CI, confidence interval; FPIA, fluorescence polarization immunoassay.
The metabolite 3-hydroxy-MEGX, which cross-reacts in the fluorescence polarization immunoassay, is a minor metabolite in humans (11), but it can make a major contribution to lidocaine metabolites in some animal models (12).
This last technique was then extended to ELISA (11) and fluorescence polarization immunoassay (12).
Although a fluorescence polarization immunoassay (FPIA) for plasma tHcy has been developed (10,11) and is now widely used in clinical laboratories, we have sought the development of an alternative method for the determination of tHcy that takes advantage of the analytical versatility, specificity, and sensitivity unique to the combination of stable isotope dilution and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) (12).
Therefore, the recently described enzyme-linked immunoassay (EIA) (13) and fluorescence polarization immunoassay (FPIA) methods (14) for plasma tHcy may become popular with laboratories that offer this assay.
This IMx Homocysteine assay (Abbott Laboratories) is a fluorescence polarization immunoassay for the quantitative measurement of tHcy in human plasma and serum with no manual sample pretreatment (5).
A new pretreatment reagent for the Emit[R] (Emit-NPT) (7) and two new automated monoclonal immunoassays are presently being introduced: a fluorescence polarization immunoassay on the AxSYM[R] instrument from Abbott (8) and a cloned enzyme donor immunotechnique (CEDIA[R]) from Boehringer Mannheim.
The most commonly used commercial MTX assays are antibody-based immunoassays such as the fluorescence polarization immunoassay (FPIA; Abbott Laboratories) and the enzyme-multiplied immunoassay (Emit; Behring Diagnostics, Syva Business) (11-14).

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