A significant apparent digoxin concentration was observed both in vitro and in vivo using the fluorescence polarization immunoassay, but no apparent digoxin concentration was observed using enzyme-linked chemiluminescent immunosorbent digoxin assay and turbidimetric assay.
3) It has been previously reported that Asian and American ginsengs and Ashwagandha (Indian ginseng) interfere with serum digoxin measurement by the fluorescence polarization immunoassay (FPIA).
The fluorescence polarization immunoassay kits for digoxin were purchased from the Abbott Laboratories (Abbott Park, Ill) and the assays were run on an FLx/TDx analyzer (Abbott).
4] Nonstandard abbreviations: tHcy, total homocyst(e)ine; SBDF, ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate; GC-MS, gas chromatography-mass spectrometry; EIA, enzyme immunoassay; and FPIA, fluorescence polarization immunoassay
A new pretreatment reagent for the Emit[R] (Emit-NPT) (7) and two new automated monoclonal immunoassays are presently being introduced: a fluorescence polarization immunoassay on the AxSYM[R] instrument from Abbott (8) and a cloned enzyme donor immunotechnique (CEDIA[R]) from Boehringer Mannheim.
The Abbott Laboratories AxSYM monoclonal fluorescence polarization immunoassay and TDx-m use the same proprietary antibody.
58), who stated that specific fluorescence polarization immunoassay and RIA methods provided valid results irrespective of their cross-reactivity.
The most commonly used commercial MTX assays are antibody-based immunoassays such as the fluorescence polarization immunoassay (FPIA; Abbott Laboratories) and the enzyme-multiplied immunoassay (Emit; Behring Diagnostics, Syva Business) (11-14).
Methotrexate measurements in plasma: comparison of enzyme multiplied immunoassay technique, TDx fluorescence polarization immunoassay, and high pressure liquid chromatography.
Chemiluminescence - High Pressure Liquid Chromatography (HPLC) - Gas Chromatography/Mass Spectrometry - Liquid Chromatography/Mass Spectrometry - Flucytosine - Triazoles - Itraconazole - Voriconazole - Posaconazole - Enzyme Immunoassays - Enzyme Multiplied Immunoassay Technique - Cloned Enzyme Donor Immunoassays - Radio Immunoassays - Fluorescence Polarization Immunoassays
- Lateral Flow Immunoassays - Particle Enhanced Turbidimetric Inhibition Immunoassays - Affinity Chrome Mediated Immunoassays
The patented technology removes the molecular weight limitation of fluorescence polarization immunoassays
by using long-lived, fluorescent metal- ligand complexes as the antigen label.
Multiple factors could account for the interlaboratory variability, including differentmethodologies (most common methods are based on fluorescence polarization immunoassays
and HPLC), different approaches to test calibration, lack of availability of reference materials for the various forms of homocysteine, or varying efficiencies for the dissociation of homocysteine-containing complexes.