fluorescence microscopy


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fluorescence

 [floo͡-res´ens]
the property of emitting light while exposed to light, the wavelength of the emitted light being longer than that of the absorbed light.
fluorescence microscopy the use of a fluorescence microscope to identify microorganisms or specific tissue constituents that have been stained with a fluorochrome or a fluorochrome-labeled substance (such as an antibody to a tissue antigen). A fluorescent antibody test can be used in place of time-consuming culture methods for identifying bacteria. See also immunofluorescence.

microscopy

 [mi-kros´kah-pe]
examination with a microscope.
fluorescence microscopy conjugation of antibodies with fluorescent dyes in order to identify specific microorganisms or tissue constituents; see also fluorescence microscopy.

fluor·es·cence mi·cros·co·py

a procedure based on the fact that fluorescent materials emit visible light when they are irradiated with ultraviolet or violet-blue visible rays; some materials manifest this property naturally, whereas others may be treated with fluorescent solutions (somewhat analogous to staining); when the absorption of the specimen is in the relatively long ultraviolet range a filter that transmits these radiations is used, and a yellow filter is placed on or in the ocular; the background field is then dark, and any yellow or red fluorescence becomes visible.

fluorescence microscopy

n.
Microscopy using naturally fluorescent or treated materials that emit visible light when they are irradiated with ultraviolet or violet-blue visible rays.

fluor·es·cence mi·cros·co·py

(flōr-es'ĕns mī-kros'kŏ-pē)
A procedure based on the fact that fluorescent materials emit visible light when they are irradiated with ultraviolet or violet-blue visible rays; some materials manifest this property naturally, whereas others may be treated with fluorescent solutions (somewhat analogous to staining).

fluorescence microscopy

a method of microscopy in which fluorescent dyes are used to mark certain structures (e.g. nucleic acids) which can then be viewed with an ultraviolet microscope.

fluorescence

the property of emitting light while exposed to light, the wavelength of the emitted light being longer than that of the absorbed light.

fluorescence-activated cell sorter (FACS)
an instrument for analysis (FACscan) and separating mixed populations of cells after labeling individual cell-specific surface antigens with fluorescent antibody. The individual cells in droplets are passed through a laser beam; the droplet is deflected into one of two or more collection vessels depending upon which fluorescent antibody is bound to its surface. Two or more different fluorescent antibodies are used.
fluorescence microscopy
the use of techniques for conjugating antibodies with fluorescent dyes in order to identify specific microorganisms or tissue constituents using a fluorescence microscope. Fluorescent antibody (FA) techniques can be used in place of time-consuming culture methods for identifying bacteria and viruses. There are two major types of FA techniques, direct and indirect, both of which are based on the antigen-antibody reaction in which the antibody attaches itself to its specific antigen.
In the direct fluorescent antibody (DFA) method, the antibody is bound to the antigen, for example, a bacterial cell in a smear, and cannot be easily removed by elution (washing). The antibody remains attached to the cell after all other serum proteins have been washed away. Since the antibody has been rendered fluorescent by conjugation with fluorescein or another dye, the outline of the bacterial cell that it coats can readily be seen with a special microscope.
In the indirect method (IFA), the specific antibody is allowed to react with the antigen. The slide is then washed and treated with a labeled antibody to the specific antibody. For example, if the specific antibody was raised in a rabbit, it is then treated with fluorescein-labeled anti-rabbit globulin, which results in a combination of this labeled antibody with the rabbit immunoglobulin already attached to the antigen.
Fluorescent antibody studies have been used in the detection of numerous bacterial, viral, fungal and protozoan infections and in the identification and localization of many tissue antigens.
References in periodicals archive ?
Table 1--Comparison of ZN smear microscopy results and LED fluorescence microscopy results with culture results.
Comparing phototoxicity during the development of a zebrafish craniofacial bone using confocal and light sheet fluorescence microscopy techniques.
The company manufactures a line of complete blood count analyzers and fluorescence microscopy products.
The importance of fluorescence microscopy was recently recognized with the 2008 Nobel Prize for Chemistry, which was awarded for the development of the green fluorescent protein (GFP), which has played a crucial role in our identification and understanding of proteins.
Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy
With their values for dark noise (3 electrons) and quantum efficiency (over 80%), these rolling shutter models offer a price-effective option for manual light or fluorescence microscopy.
In recent years, CLEM techniques, which correlate electron microscopy with fluorescence microscopy, have been developed.
CoolLED designs, manufactures and markets illumination systems for fluorescence microscopy.
The cases make use of Western Blots, scratch wound assays, bright field microscopy, and fluorescence microscopy.
Fluorescence microscopy techniques cause molecules that fluoresce to light up when illuminated with excitation light.
Economical, reliable and affordable, the illuminators are easy-to-use in fluorescence microscopy applications.
19 -- Carl Zeiss MicroImaging has incorporated new LED technology in the Colibri illumination system, a light source system for wide field fluorescence microscopy that uses specific wavelength windows with much less need to suppress unwanted peripheral wavelengths from a white light arc lamp.

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