The option of having flanking regions
present or absent in the expressed ssDNA product is an additional useful property of this work.
Variability in the physicochemical characteristics of the amplified sequences, GC content, flanking regions
and secondary structures also may add to the imbalance of the reaction and the subsequent outcomes.
FJ868583), 1,239 bp of flaB and flanking regions
(GenBank accession no.
We determined MCP DNA sequences of 1,472 nt that included the complete coding region (nt positions 17-1408, 1,392 nt) and proximal flanking regions
These data suggest that during horizontal transfer between species and genera, the association of floR with its flanking regions
has been conserved (5,6).
CTX-M-15] was investigated by sequencing the flanking regions
The nucleotide sequence of the strA and strB genes and of flanking regions
from pIP1203 has been deposited in the EMBL data bank under accession number AJ249779.
To confirm the PERV integrations, both the 5' and 3' flanking regions
for each PERV were amplified by polymerase chain reaction (PCR).
In addition to the coding region, more than 400bp of 5' flanking, and 1200bp of 3' flanking regions
We found that 15 of the 21 exons of the ATP7B gene have SNPs within 250 by of their 5' and 3' flanking regions
in the NCBI human genome database (Fig.
The catalytic core is made sequence specific by two flanking regions
that will pair in a complementary manner with nucleotides 5' and 3' of the target site.
6,204,027, "Ribozymes Having 2'-O-substituted Nucleotides in the Flanking Sequences," owned by the University of Massachusetts and exclusively licensed to Hybridon, contains claims covering hammerhead and hairpin ribozymes comprising one or more 2'-O-methyl-substituted nucleotides in one or both of the ribozyme's flanking regions