Basically, similar corrections are made, such as those made to convert directly read ISE results to values obtained with flame photometry
Its 29 chapters covered principles of spectrophotometry, flame photometry
, gasometric techniques, electrophoresis, accuracy, precision, control charts, significant figures (still abused by most authors and journals), and normal values.
Corrections were carried out by introduction of slope and intercept values established with the flame photometry
reference method (Eppendorf apparatus) for calibration of each new batch of slides.
Similarly, the introduction of flame photometry
and its rapid and precise estimation of serum K
1) Evaluation: In the past, when we moved from chemical to enzymatic determinations or from flame photometry
to ion-selective electrodes, groups of clinical chemists designed adapted evaluation protocols that allowed the organization of comparative evaluations to get a consensus on technical specifications .
Use of more-dilute (1000 units/mL) heparin solution, or flame photometry
measurement of sodium (an indirect method as on the Hitachi), showed no negative interference .
But we can and should teach them all the underlying principles of spectrophotometry, fluorometry, flame photometry
, nephelometry/turbidimetry, and ion-selective electrodes, and relate how these principles apply to the instruments used in laboratories today.