flame photometry


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flame photometry

Etymology: L, flagrare, to burn; Gk, phos, light, metron, measure
measurement of the wavelength of light rays emitted by excited metallic electrons exposed to the heat energy of a flame, used to identify characteristics in clinical specimens of body fluids. The intensity of the emitted light is proportional to the concentration of atoms in the fluid, and a quantitative analysis can be made on that basis. In the clinical laboratory, flame photometry was once used to measure sodium, potassium, and lithium levels but is no longer used routinely.
References in periodicals archive ?
Basically, similar corrections are made, such as those made to convert directly read ISE results to values obtained with flame photometry (7-9).
Its 29 chapters covered principles of spectrophotometry, flame photometry, gasometric techniques, electrophoresis, accuracy, precision, control charts, significant figures (still abused by most authors and journals), and normal values.
Corrections were carried out by introduction of slope and intercept values established with the flame photometry reference method (Eppendorf apparatus) for calibration of each new batch of slides.
Similarly, the introduction of flame photometry and its rapid and precise estimation of serum K
1) Evaluation: In the past, when we moved from chemical to enzymatic determinations or from flame photometry to ion-selective electrodes, groups of clinical chemists designed adapted evaluation protocols that allowed the organization of comparative evaluations to get a consensus on technical specifications [9].
Use of more-dilute (1000 units/mL) heparin solution, or flame photometry measurement of sodium (an indirect method as on the Hitachi), showed no negative interference [1].
But we can and should teach them all the underlying principles of spectrophotometry, fluorometry, flame photometry, nephelometry/turbidimetry, and ion-selective electrodes, and relate how these principles apply to the instruments used in laboratories today.