Polymerase chain reaction primers and probes derived from flagellin
gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever.
Physically linking flagellin
to antigens leads to a more potent vaccine than just administering a mixture of the two unattached components.
In another set of experiment FLS from 4 patients with JIA and two patients with RA were stimulated with 5 [micro]g/ml of flagellin
(Invivogen, San Diego, CA, USA), 20 [micro]g/ml of poly I:C (Invivogen, San Diego, CA, USA) and 10 [micro]g/ml of imiquimod (Invivogen, San Diego, CA, USA) in 24 well culture plates for 48 h.
Because Borrelia specific primers were used for the flagellin
gene where B.
is a dominant antigen in Crohn disease.
Previous genotyping studies employing the polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) typing, based on the flagellin
(flaA) gene have been unable to generate an amplicon for the urease-positive thermophilic Campylobacter (UPTC), which are the predominant taxa associated with shellfish, largely caused by sequence diversity between the UPTC group and C.
Earlier this year, scientists in Switzerland documented a defensive chemical cascade unleashed when some plants detect flagellin
, a protein in the propulsive fibers of some bacteria.
Three bacterial proteins, flagellin
(41 kD) OSP B (35 kD), and OSP A (31 kD) used as an indication of antibody specificity for B.
Remarkable differences exist between strains in their ability to produce key virulence factors like flagellin
and glucosylated polysaccharides.
Specifically, "up-regulated" virulence factors identified in NST-grown bacteria so far include Type III protein translocation system substrates, flagellin
expression, glycosylation of bacterial proteins, hemolysin and capsular polysaccharide production, and lipopolysaccharide structure.
VAX161 is a recombinant fusion protein that consists of the globular head domain of the hemagglutinin antigen (HA) of influenza A HA5 Indonesia fused to Salmonella typhimurium flagellin
type 2 (STF2), a toll-like receptor 5 (TLR5) ligand.
a 766-bp region of the flagellin
gene sequence were shown to have and a 0.