Commonly used additives in the maintenance cell culture medium are fetal calf
serum (FCS), goat serum, horse serum, allontoic fluid, colostral whey, oval albumin, etc.
02 IU/mL FSH, 1 u g/mL estradiol-17b (E2), 50 u g/mL gentamicin and 1) 10% fetal calf
serum (FCS), 2) 6 mg/ml FAF-BSA or 3) 6 mg/ml FAF-BSA + 10.
For immunoblot procedures, the particular recombinant antibodies were diluted using DMEM supplemented with 100 mL/L heat-inactivated fetal calf
serum and applied to the corresponding AlaBLOTs (F1, hen egg white; F2, bovine milk white; 11, whole bee venom; and F49, apple extract; DPC-Biermann) with native allergens, separated by SDS-PAGE, and immobilized onto nitrocellulose strips in the form of extracts or purified allergens.
For experiments, cells were subcultured into gelatin-coated 12-well plates in DMEM medium supplemented with glutamine (1 mM), penicillin (100 IU/ml), 10% fetal calf
serum and 10% human serum until subconfluency and confluency, in separated cultures, was reached.
In 2004, an atypical pestivirus, D32/00_HoBi, distantly related to BVDV-1 and BVDV-2 was isolated from a batch of fetal calf
serum collected in Brazil (3).
Aortic hVSMCs (Cambrex Bioscience Srl) cultured in minimum essential medium supplemented with 100 mL/L fetal calf
serum, 100 units/L penicillin, 100 [micro]g/L streptomycin, 10 mmol/L glutamine, and vitamins and buffered with 10 mmol/L TES and 10 mmol/L HEPES were incubated for 24 h with sodium azide at a final concentration of 76.
Cells were then centrifuged, resuspended in RPMI 1640 culture medium supplemented with 10% fetal calf
serum, 200 U/mL penicillin, 0.
5]) (Kiderlen and Kaye, 1990) in 200 [micro]l RPMI 1640 medium supplemented with 5% heat-inactivated fetal calf
serum (herein designated R5) were incubated for 48 h with test compounds (50 [micro]g/ml) or medium alone before further fetal calf
serum (10% final concentration) was added.
2 in BHK-21 cell line having Glasgow Minimal Essential Medium (GMEM) without Fetal Calf
After incubation for 24 h, monolayers of ECV304 were maintained in M199 medium containing 10% fetal calf
serum and observed daily with an inverted microscope.
Different amounts of testosterone and other androgenic compounds were added to charcoal-stripped fetal calf
serum, and the corresponding dose-response curves were obtained and compared with that of testosterone (Fig.
Cells were grown in phenol red-free EMEM containing 2 mM glutamine, 1% nonessential amino acids, 100 U/mL antibiotics (mix of penicillin, streptomycin, and fungizone), 1 mM sodium pyruvate, and 10% fetal calf
serum (Biowhittaker, Gagny, France).