To ensure the iron deprived condition, firstly we did
ferroxidase enzymatic assay to estimate the non-oxidized ferrous iron present in the reaction mixture.
Determination of ceruloplasmin content by
ferroxidase activity assay
Serum
ferroxidase activity in patients with multiple sclerosis: A Pilot Study," In Vivo, vol.
Serum
ferroxidase albumin ratio as a marker in pulmonary tuberculosis.
We previously developed an immunoassay for CP, a
ferroxidase that plays a role in copper and iron metabolism, and characterized the antibody pair for this blood protein (15).
Ceruloplasmin is copper carrying protein which functions as a
ferroxidase and superoxidase scavenger.
Ceruloplasmin is an [alpha]2-glycoprotein synthesized in hepatocytes that binds plasma copper and has
ferroxidase activity.
It is remarkably similar to 1-I-ferritin that assembles into typical ferritin shells within mitochondria and has
ferroxidase activity (41).
CP also has an important role as a
ferroxidase and superoxide scavenger.
Assessment of
ferroxidase and radical scavenging activities.
The links between APP and metal metabolism are further emphasized by the interaction of APP with ferroportin, to promote iron export and its
ferroxidase activity [92, 93].
ceruloplasmin) CP Copper-binding (6-7 atoms per molecule) glycoprotein with
ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species.