Total SOD activity was determined by monitoring the inhibition of the reduction of ferricytochrome
C at 550 nm, using the xanthine-xanthine oxidase system as the source of superoxide.
UQCRC1 is an oligomeric enzyme that catalyzes the transfer of electrons from coenzyme QH2 to ferricytochrome
c with the coupled translocation of protons across the mitochondrial inner membrane (Brandt and Trumpower, 1994).
Reduction of ferricytochrome c by superoxide was monitored from the increase in absorbance at 550 nm with reference at 540 nm for 45 minutes .
Kinetics and mechanism of the reduction of ferricytochrome c by the superoxide anion.
2], and the start reagent 2 vessel contained ferricytochrome
2] determination was based on reduction of ferricytochrome
The superoxide-scavenging activity was calculated by measuring the reduction rate of ferricytochrome