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extragenic |
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extragenic occurring outside a gene, or in a gene other than the one in question. |
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| The following techniques have been used for this purpose: pulsed-field gel electrophoresis (PFGE); restriction fragment length polymorphism; enterobacterial repetitive intergenic consensus (ER1C)-PCR; repetitive extragenic palindromic (REP) PCR; infrequent restriction site (IRS)-PCR (10,13-18); DNA sequencing-based approaches represented by sequences of 16S rDNA (19,20), ftsZ (21), gltA (22), 35-kDa protein-encoding (23), groEL, and pap31 genes (24); and the 16S-23S intergenic spacer (25). The snxB1 mutation has been isolated as an extragenic suppressor of the nimX2 mutation of the filamentous fungus Aspergillus nidulans. alcohol, and drugs; 6) screens to detect altered gene expression patterns as a tool to identify components of genetic pathways or those altered by environmental agents; and 7) sensitized screens using strains carrying a known mutation to identify extragenic suppressors or enhancers of that mutation. |
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