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exonuclease
(redirected from Exonuclease III)

   Also found in: Dictionary/thesaurus, Encyclopedia, Wikipedia 0.03 sec.
exonuclease /exo·nu·cle·ase/ (ek″so-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of terminal bonds of deoxyribonucleotide or ribonucleotide chains, releasing mononucleotides.
ex·o·nu·cle·ase (ks-nkl-s, -z)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

exonuclease
[ek′sōno̅o̅′klē·ās]
Etymology: Gk, exo + L, nucleus, nut; ase, enzyme
an enzyme that digests DNA or RNA from the ends of the strands. Compare endonuclease.

exonuclease [ek″so-noo´kle-ās]
an enzyme that cleaves single mononucleotides from the end of a polynucleotide chain.

exonuclease
a nuclease that cleaves single mononucleotides from the end of a polynucleotide chain.

exonuclease III
one from E. coli that removes nucleotides from the 3′ ends of double-stranded DNA.


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Removal by human apurinic/apyrimidinic endonuclease 1 (Ape 1) and Escherichia coli exonuclease III of 3¶-phosphoglycolates from DNA treated with neocarzinostatin, calicheamicin and g-radiation.
Materials and Methods Reagents Recombinant, untagged human Ape1, Escherichia coli exonuclease III (ExoIII), and E.
The presence of Exonuclease III reduces the final overall yield of amplified material at endpoint and so is not suitable for analysis on gels, however it is the preferred system for generating strong fluorescence signal kinetics in the RPA system.
 
 
 
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