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exonuclease |
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exonuclease /exo·nu·cle·ase/ (ek″so-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of terminal bonds of deoxyribonucleotide or ribonucleotide chains, releasing mononucleotides.
exonuclease [ek′sōno̅o̅′klē·ās] Etymology: Gk, exo + L, nucleus, nut; ase, enzyme an enzyme that digests DNA or RNA from the ends of the strands. Compare endonuclease. exonuclease a nuclease that cleaves single mononucleotides from the end of a polynucleotide chain. exonuclease III one from E. coli that removes nucleotides from the 3′ ends of double-stranded DNA. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| The probe, which contains both a fluorescence reporter dye at the 5'-end (6-carboxyfluorescein, 6-FAM: maximum emission wavelength = 518 nm) and a quencher dye at the 3'-end (6-carboxytetramethyl rhodamine, TAMRA: maximum emission wavelength = 582 nm), is degraded by the 5'-3' exonuclease activity of the Taq DNA polymerase, and the resulting fluorescence is detected by a laser in the sequence detector (TaqMan ABI Prism 7700 Sequence Detector System; PerkinElmer). 129 BEESE LS,EMBO J,vol 0010,page 0025,1991,cites= 59,STRUCTURAL BASIS FOR THE 3$-5$ EXONUCLEASE ACTIVITY OF ESCHERICHIA-COLI DNA-POLYMERASE-I - A 2 METAL-ION MECHANISM From the structure of one such enzyme, which they describe in the March 23 Nature, these researchers conclude that an exonuclease may recognize a nucleotide that needs pruning by flipping out the "orphan" base--the one opposite the damaged site--and binding to that. |
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