exonuclease


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exonuclease

 [ek″so-noo´kle-ās]
an enzyme that cleaves single mononucleotides from the end of a polynucleotide chain.

ex·o·nu·cle·ase

(ek'sō-nū'klē-ās),
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid); several have been prepared from Escherichia coli, designated exonuclease I, exonuclease II, etc.; exonuclease III, which removes nucleotides from 3' ends of DNA, is used in DNA sequencing. Compare: endonuclease.

exonuclease

/exo·nu·cle·ase/ (ek″so-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of terminal bonds of deoxyribonucleotide or ribonucleotide chains, releasing mononucleotides.

exonuclease

(ĕk′sō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

exonuclease

[ek′sōno̅o̅′klē·ās]
Etymology: Gk, exo + L, nucleus, nut; ase, enzyme
an enzyme that digests DNA or RNA from the ends of the strands. Compare endonuclease.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease

exonuclease

an enzyme that removes a terminal NUCLEOTIDE (3' or 5') in a POLYNUCLEOTIDE CHAIN. Exonucleases remove the nucleotides in a successive way, one by one, and are highly specific in their action.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease

exonuclease

a nuclease that cleaves single mononucleotides from the end of a polynucleotide chain.

exonuclease III
one from E. coli that removes nucleotides from the 3′ ends of double-stranded DNA.
References in periodicals archive ?
The most obvious of these is the nearly immediate degradation of normal phosphodiester-linked nucleotides by the exonucleases abundant both in serum and within cells.
Cellectis's teams thus succeeded in improving current genome engineering techniques by employing a new approach: co-expressing meganucleases or TALENs[TM], Cellectis's internal technology, together with exonucleases.
DNA polymerase s and 8 exonuclease domain mutations in endometrial cancer.
Reaching the annealed probe, the 5' [right arrow] 3' exonuclease capable DNA polymerase breaks the probe apart, physically releasing the reporter dye from its quencher partner.
Redundant exonuclease involvement in Escherichia coli methyl-directed mismatch repair.
Telomeres therefore serve to protect chromosomes and maintain genomic integrity from recombination, exonuclease degradation and end-to-end fusion (Blackburn et al.
The 3o-5o exonuclease activity of Deep Vent increases the fidelity and robustness of Taq, while the optimized buffers further enhance yield and product purity, regardless of a template's GC content.
The 5'-labeled MGB probes are resistant to the polymerase exonuclease activity.
A similar inhibition pattern was observed with the homologous Eseheriehia coil protein, exonuclease III, but no inhibition was seen with the structurally distinct AP endonuclease E.
The probe is partially degraded by the polymerase's exonuclease activity in a template-specific reaction, and the product triggers the cleavage of a universal fluorogenic probe.
It is a family-A DNA polymerase, with both DNA polymerase activity and exonuclease proofreading activity.
The genomic DNA is then converted to single-stranded targets by using multiplex polymerase chain reaction (PCR) amplification and exonuclease digestion.