We used PCR to detect the presence of the PVL- and toxic shock syndrome toxin 1--encoding genes (lukS-lukF PV and tst, respectively), exfoliatin
A--and B--encoding genes (eta and etb, respectively) (7), and enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sem, sen, seo, sep, seq, ser) as described by Omoe et al.
Bark of three year old twigs rough, exfoliatin
Multiplex PCR was used to test for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliatin A and B genes (6).
Genes encoding toxic shock syndrome toxin 1 (n = 4) and exfoliatin A (n = 3) were detected in 7 MSSA isolates.
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is responsible for severe infections related to carriage of exotoxins such as the Panton-Valentine leukocidin (PVL), toxic shock syndrome toxin 1 (TSST-1), or exfoliatin A (1).
Presence of type V cassette, TSST-1, and exfoliatin toxins was assessed by using specific oligonucleotides (sequences are available at www.
A total of 92 isolates (61%) harbored at least 1 toxin gene, most frequently PVL (n = 60), followed by TSST-1 (n = 22) and exfoliatin A (n = 11).
coagulase, hemolysis [a (Hla), b, g (HLG), d], enterotoxins, toxic shock syndrome toxin (TST), exfoliatins
and PVL) (Tomita and Kamio, 1997; Archer, 1998).
Comparison of the prevalence of genes coding for enterotoxins, exfoliatins
, panton-valentine leukocidin and tsst-1 between methicillin-resistant and methicillin-susceptible isolates of Staphylococcus aureus at the university hospital in olomouc.