Simultaneous OF testosterone, epitestosterone, dihydrotestosterone, DHEA, androsterone, etiocholanolone
, and [alpha]- and [beta]-androstanediol quantification collected with the Quantisal device were presented, but never published (40).
Saliva requires careful Etiocholanolone
attention to collection procedures to avoid falsely elevated testosterone levels.
etiocholanolone and dimethyl sulfoxide were from Sigma Chemical Co.
The cells were counted using a hemocytometer, and the purity was assessed by histochemical staining for 3[beta]-HSD using 1 mg/ml etiocholanolone as the enzyme substrate (Klinefelter et al.
The research has revealed that in certain rare circumstances, a chemical reaction can occur in the bottle after a urine sample has been provided, whereby two endogenous hormones, androsterone (A) and etiocholanolone
(E), are converted into 19-NA and 19-NE, metabolites of nandrolone that are tested for in doping analysis.
Androgen metabolites are analyzed separately by sex, since male androgen metabolites in this age group are increased from testicular-derived androsterone and etiocholanolone
13]C values for acetylated androsterone and etiocholanolone as described previously (13).
13]C VALUES FOR ACETYLATED ANDROSTERONE AND ETIOCHOLANOLONE FROM ATHLETES' URINES WITH HIGH EPITESTOSTERONE CONCENTRATIONS
Testosterone, testosterone glucuronide (TG), androsterone, androsterone glucuronide (AG), etiocholanolone
glucuronide (EtG), 17[alpha]-hydroxyprogesterone (17[alpha]OHP), and Escherichia coli [beta]-glucuronidase were purchased from Sigma.
The binding of androsterone sulfate, etiocholanolone
sulfate, and dehydroepiandrosterone sulfate by human plasma protein.
, 11[beta]-hydroxyandrosterone, 11[beta]-hydroxyetiocholanolone, androst-5-en-3,17-diol, 5a-androstan-3a,17[beta]-diol, 5[beta]-androstan-3a,17[beta]-diol, dihydrotestosterone, DHEA, T, and E were purchased from either Sigma Chemical Co.
Urinary androsterone and etiocholanolone
account for ~70% of a dose of T ; however, many other endogenous steroids are metabolized to androsterone and etiocholanolone
, and neither their concentrations nor their ratio is a useful marker of T administration.