equilibrium dialysis


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e·qui·lib·ri·um di·al·y·sis

in immunology, a method for determination of association constants for hapten-antibody reactions in a system in which the hapten (dialyzable) and antibody (nondialyzable) solutions are separated by semipermeable membranes. Because, at equilibrium the quantity of free hapten will be the same in the two compartments, quantitative determinations can be made of hapten-bound antibody, free antibody, and free hapten.

e·qui·lib·ri·um di·a·ly·sis

(ē'kwi-lib'rē-ŭm dī-al'i-sis)
immunology A method for determination of association constants for hapten-antibody reactions in a system in which the hapten (dialyzable) and antibody (nondialyzable) solutions are separated by semipermeable membranes.

equilibrium

a state of balance between opposing forces or influences. In the body, equilibrium may be chemical or physical. A state of chemical equilibrium is reached when the body tissues contain the proper proportions of various salts and water. See also acid-base balance and fluid balance. Physical equilibrium, such as the state of balance required for walking or standing, is achieved by a very complex interplay of opposing sets of muscles. The labyrinth of the inner ear contains the semicircular canals, or organs of balance, and relays to the brain information about the body's position and also the direction of body motions. Genetic equilibrium is achieved when the allelic frequencies do not change from generation to generation.

equilibrium dialysis
a technique for determining the affinity of an antibody for an antigen.
equilibrium disturbances
see posture, posture balance.
dynamic equilibrium
the condition of balance between varying, shifting and opposing forces that is characteristic of living processes.
References in periodicals archive ?
Free thryroxine, measured by equilibrium dialysis, avoids this laboratory testing inaccuracy.
Accurate free thyroxine (FT4) quantitation in human serum by combined equilibrium dialysis and direct mass spectrometry measurement.
a) Immunoassay system Principle Immulite 2500 1-Step chemiluminescent assay Advia Centaur 1-Step chemiluminescent assay TOSOH AIA 1800 1-Step fluoroimmunoassay Elecsys E170 1-Step (2-step incubation) Chemiluminescent assay Architect 2-Step chemiluminescent assay Beckman DXI 800 2-Step chemiluminescent assay Wallac Delfia 2-step assay Nichol FT4 Equilibrium dialysis Physical separation of free hormone, then immunoassay Immunoassay system TSH, mU/L FT4, pmol/L Immulite 2500 0.
Free thyroid hormones in serum by direct equilibrium dialysis and online solidphase extraction-liquid chromatography/tandem mass spectrometry.
New ultrafiltration method for free thyroxin compared with equilibrium dialysis in patients with thyroid dysfunction and nonthyroidal illness.
These investigators dissected one analog-based free T assay by measuring total T and free T by analog methods both in the retentate and dialysate fractions of serum after equilibrium dialysis.
1) during equilibrium dialysis at 37 [degrees]C by HEPES acid in the dialysate buffer (14).
Finally, although ultrafiltration, equilibrium dialysis, and direct analog assays have the same aims, in regard to sampling and dilution these different assays represent opposite ends of a spectrum of valid methods.

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