Fluorescent labeling of cells proved that both cell types attached with simultaneous seeding of both epitenon and endotenon cells.
In the future, adding growth factors or adhesion proteins to manipulate the seeding process may improve the spatial orientation of the epitenon and endotenon cells.
Zone II flexor tendon defects may be bridged by the use of an allogenic acellularized intrasynovial tendon scaffold repopulated with intrasynovial epitenon and endotenon cells.
Both epitenon and endotenon cells were successfully labeled with green and red fluorescent markers, respectively.
As previously discussed, intrasynovial tendons with their lining of epitenon cells have proven superior to extrasynovial tendons with respect to tendon healing.
The intrasynovial tendons are lined with only a single layer of epitenon cells.
As for flexor tendon tissue engineering, epitenon and endotenon cells are logical candidates.
25 percent Trypsin at 37[degrees]C for 20 minutes to release the epitenon tenocytes.
Nanotopographical responsiveness has been observed in diverse cell types including fibroblasts, osteoblasts, osteoclasts, endothelial, smooth muscle, epithelial, and epitenon