Alkaline phosphatase staining of pig and sheep epiblast
cells in culture.
Derivation of pluripotent epiblast
stem cells from mammalian embryos.
Embryonic stem cells come from a class of tissue, the epiblast
, in the inner cell mass of early stage embryos.
Those who had taken their question to the mufti had to tell him that the embryonic stem cell lines (ES cell lines) are cultures of cells derived from the epiblast
tissue of the inner cell mass (ICM) of a blastocyst--an early stage embryo--approximately four to five days old in humans and consisting of 50 to 150 cells.
stem cells were hailed last night as "the missing link" that could lead to cures for diseases such as cystic fibrosis.
Gastrulation begins dorsally with involution of deep blastoderm cells to form an outer epiblast
and inner hypoblast.
4 kb inversion in the chloroplast genome in Joinvillea and the grasses; reduction to 1 ovule per pistil, gain of a lateral "grass-type" embryo, and gain of an inversion around the gene trnT in the chloroplast genome in the grasses; loss of arm cells in the clade that consists of Brachyelytrum, Pooideae, and the PACC clade; loss of the epiblast
and gain of an elongate mesocotyl internode in the PACC clade; gain of proximal female-sterile florets in female-fertile spikelets, gain of overlapping embryonic leaf margins, and gain of Panicum-type endosperm starch grains in the clade that comprises Centothecoideae and Panicoideae; and loss of the scutellar tail of the embryo in Pooideae (in one of two alternative placements of Pooideae among other groups).
We previously conducted a comparative study of stem cells reprogrammed in pigs using epiblast
stem cell (EpiSC)-like pESC lines derived from porcine embryos of various origins, including in vitro fertilized (IVF), in vivo-derived, IVF-aggregated, and parthenogenetic embryos (Park et al.
The mouse cells that we utilized, which are pluripotent epiblast
stem cells, can make any cell type in body," explained Paul Tesar, an assistant professor of genetics at Case Western Reserve and senior author of the stud.
Stern hypothesizes that local movement in the epiblast
, the epithelial cells, are "responsible for positioning and shaping the primitive streak" by pushing in among neighboring cells and this "can also explain the polonaise movements without the need for long-range gradients" (p.