enzyme-multiplied immunoassay technique

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Related to enzyme-multiplied immunoassay technique: fluorescence polarization immunoassay

en·zyme-mul·ti·plied im·mu·no·as·say tech·nique (EMIT),

a type of immunoassay in which the ligand is labeled with an enzyme, and the enzyme-ligand-antibody complex is enzymatically inactive, allowing quantitation of unlabeled ligand.
See also: competitive binding assay, enzyme-linked immunosorbent assay.

en·zyme-mul·ti·plied im·mu·no·as·say tech·nique

(EMIT) (en'zīm-mŭl'ti-plīd im'yū-nō-as'ā tek-nēk')
A type of test in which the ligand is labeled with an enzyme.
See also: competitive binding assay, enzyme-linked immunosorbent assay

enzyme-multiplied immunoassay technique

Abbreviation: EMIT
An enzyme immunoassay based on a mixture of analyte and enzyme substrate such that no immobile phase is necessary.
See: enzyme immunoassay; cloned enzyme donor immunoassay
See also: technique
References in periodicals archive ?
The team compared the results with those obtained through a technique using real antibodies, called enzyme-multiplied immunoassay technique (EMIT).
This may be similar to the reported masking effects of benzalkonium chloride (Visine[TM]) with the enzyme-multiplied immunoassay technique and TDx[TM] (Abbott Laboratories) assays for 9-carboxy-tetrahydrocannabinol; the drug is proposed to partition into micelles formed by benzalkonium chloride so that it is not accessible to antibodies (3).
We used both an enzyme-multiplied immunoassay technique (EMIT; Behring) and a monoclonal fluorescence polarization immunoassay (mFPIA; Abbott) to assay in parallel 163 EDTA-anticoagulated blood samples from 87 heart transplant patients.

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