To investigate the possibility of an integration of an REV gene sequence into the FWPV genome, we designed another 2 sets of primers for the amplification of a partial REV env gene
and the REV env-FWPV open reading frame 203, which contains the entire REV 3' long terminal repeat.
Internal PERV control primers were designed to amplify conserved regions between 5' long terminal repeat (LTR) and 3' env gene
The assay utilizes PCR-amplified env gene
and hybridizes those genes to V3-coding sequences from viruses with known tropisms.
They examined the most variable region of the virus' env gene
, which encodes a protein forming the outer coat of the virus.
The env gene
encodes an HIV coat protein that allows the virus to recognize and attach to human cells.
Numerous laboratories have reported that a gene segment that corresponds to a unique 660 base pair sequence of the MMTV envelope gene, known as the env gene
, is present in 30%-40% of fresh and archival breast cancer specimens from patients in the United States, Italy, Mexico, Brazil, and Argentina; 80% in Tunisia; and 10%-12% in China and Japan.
5) In addition, we have found MMTV-like ENV gene
sequences in non-Hodgkin lymphomas (NHL) of 3 of 19 patients diagnosed with only lymphoma.
The viral vaccines of the invention are optimally combined with a recombinant HIV env booster, or a recombinant HIV env gene
DNA priming or boosting vaccine.
43) analyzed proviral env gene
sequences (V1, V2, and V3) in infected cells obtained from cervical secretions and PBMCs.
Like similar genes in other viruses, MMTV's env gene
encodes a protein that helps form the outer surface of the virus.
Within the env gene
and long terminal repeat regions, for example, 7%-10% divergence has been demonstrated at the nucleotide level (5,6).
The env gene
codes for a protein on the outer coat of the virus that allows it to recognize and attach to human cells.