Matrigel is a suitable matrix that includes laminins, collagen IV, entactin
, heparan sulfate proteoglycan, as well as various growth factors, which can highly representative of the tumor microenvironment.
The STBM comprises extracellular matrix (ECM) proteins, such as laminin, collagen IV, perlecan, and entactin
(Yazama et al.
The ECM gel was prepared from Engelbreth-Holm-Swarm (EHS) mouse sarcoma and was composed of laminin as the major component, collagen type IV, heparan sulfate proteoglycan, entactin
and other minor components.
These enzymes are able to split denatured collagens, fibronectin, lamin, entactin
, elastin, etc.
5) MMP-7 displays strong proteolytic activity against a wide range of ECM components including collagen types IV, V, IX, and X, proteoglycans, fibronectin, laminin, gelatin, elastin, and entactin
The proteolytic effects are seen at the level of the constituents of the extracellular matrix such as fibronectin, fibrinogen, entactin
, and heparin-sulphate proteoglycans .
Table-5: Insoluble inductive signals from extra cellular matrix Peptide Sequences Parent protein Refernces RGD Fibronectin, Collagen, Fibrinogen, 127, 128 Laminin, Vitronectin, von Willebrand factor, Entactin
, Tenascin, Thrombospondin YIGSR Laminin 129, 130 IKVAV Laminin 131, 132 LRE Laminin 133, 134 REDV Fibronectin 135, 136 DGEA Collagen 137, 138 GXG Thrombospondin 139, 140 VGVAPG Elastin 141, 142 Table-6: Effect of functional groups Functional groups Biological effects Carbonyl, Hydroxyl, Growth of bovine aortic endothelial cells was Carboxyl, etc.
Immediately adjacent to the endothelium is the lamina rara interna that consists of loosely interwoven fibrils containing such structural proteins as entactin
, heparin sulfate proteoglycans, and laminin (Timpl, 1986).
Other functionally similar molecules include tenascin, syndecan, entactin, chondronectin, and vitronectin (e.
A molecule similar to entactin occurs in Drosophila (Bogaert et al.
Basement membranes, composed of type IV collagen, laminin, proteoglycans, entactin
and other proteins, form an anatomical barrier where cells meet connective tissue (Hellmark, Segelmark, & Wieslander, 1997).