endonucleases


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endonucleases

Enzymes that can split DNA or RNA at any point along the molecule by cutting PHOSPHODIESTER BONDS.
References in periodicals archive ?
The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites.
The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations.
Protocol used in this study was based on a specific amplification of PCR product from the gene encoding the cytochromeb (a specific part of mitochondrial DNA) and on a restriction enzyme analysis with endonucleases.
Enzyme digestion was performed by HpaII, a methylation-sensitive restriction endonuclease that recognized and cut the DNA sequence CCGG and whose action would be blocked by CpG methylation.
Slides were aged during 6 h, in a dry incubator at 65[degrees]C, before the restriction endonuclease treatment.
Although both restriction endonucleases and capillary electrophoresis have been used for a wide range of applications, their combination for genotyping is not very common.
380 was digested with approximately 30 different restriction endonucleases (New England Biolabs; Promega) according to the manufacturer's instruction.
Indexers used in this study were double-stranded adaptors composed of a 20-nt common indexer primer annealed to 1 of the 256 individually synthesized 24-nt indexer strands with 4-nt 5'-protruding ends complementary to the 4-nt nonidentical 5' overhangs produced by selected type IIS restriction endonucleases.
We postulated that if AS produces oxidative DNA damage, this specific enzyme, having both DNA glycosylase and endonuclease activities, will recognize and cleave the damaged bases, and that consequently DNA strand breaks will be produced at enzyme-sensitive sites.
For multiplex analysis, PCR products were digested with restriction endonucleases (Table 1 in the Data Supplement that accompanies the online version of this article at http://www.
Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases.
These tools, based on sequence specific endonucleases, enable the engineering of cells with optimized features for drug discovery, protein production and gene function studies.