endonuclease


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endonuclease

 [en″do-noo´kle-ās]
an enzyme that cleaves internal bonds of polynucleotides.
restriction e's enzymes that cleave large DNA molecules at specific sequences of four to six nucleotides. See also recombinant dna technology.

en·do·nu·cle·ase

(en'dō-nū'klē-ās),
An enzyme (phosphodiesterase) that cleaves the internal phosphodiester bonds in a DNA molecule, thus producing DNA fragments of varying size. Compare: exonuclease.

endonuclease

/en·do·nu·cle·ase/ (-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of interior bonds of ribonucleotide or deoxyribonucleotide chains.
restriction endonuclease  an endonuclease that hydrolyzes DNA, cleaving it at an individual site of a specific base pattern.

endonuclease

(ĕn′dō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of bonds between nucleotides in the interior of a DNA or RNA molecule.

endonuclease

an enzyme that cleaves or hydrolyzes phosphodiester bonds within a polynucleotide chain. Compare exonuclease.

en·do·nu·cle·ase

(en'dō-nū'klē-ās)
A nuclease (phosphodiesterase) that cleaves polynucleotides (nucleic acids) at interior bonds, thus producing polynucleotide or oligonucleotide fragments of varying size.
Compare: exonuclease

endonuclease

a NUCLEASE that cleaves PHOSPHODIESTER BONDS from within nucleic acid molecules. See also RESTRICTION ENDONUCLEASE, compare EXONUCLEASE.

en·do·nu·cle·ase

(en'dō-nū'klē-ās)
A nuclease (phosphodiesterase) that cleaves polynucleotides (nucleic acids) at interior bonds.

endonuclease

(en´dōnoo´klēās´),
n an enzyme (nuclease) that cleaves polynucleotides at interior bonds, producing polynucleotide or oligonucleotide fragments.

endonuclease

a nuclease that cleaves internal bonds of polynucleotides.

restriction endonuclease
References in periodicals archive ?
The team led by EMBL head Stephen Cusack analyzed crystals of endonuclease from the 2009 pandemic influenza strain using the high intensity X-ray beams at the European Synchrotron Radiation Facility (ESRF).
BACKGROUND: DNA methylation analysis currently requires complex multistep procedures based on bisulfite conversion of unmethylated cytosines or on methylation-sensitive endonucleases.
After confirming that the six compounds indeed showed activity against the endonuclease, the researchers next performed a detailed structural analysis of how the compounds fit the active site.
marker 2-Overall quality of the Agarose gel electrophoresis and pDNA estimation of the ccc form and matching of the size with depository data 3-Control of the Digestion of the pDNA with a single Restriction profile endonuclease cutter and estimation of the band size Double digestion with two endonucleases that release the inserted DNA sequence of interest [cDNA] and size estimation.
Potentially useful restriction endonucleases identified by the virtual digestion analyses were then tested in a PCR/ RFLP agarose gel analysis of the ITS-1 marker in multiple individuals of each species.
Heteroduplex resolution using T7 endonuclease I in microbial community analyses.
Hae III restriction endonuclease analysis of PCR product revealed three different banding patterns consisting of 2-3 DNA fragments ranging from 0.
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases.
Uma endonuclease de restricao corta enzimaticamente ("digere") o DNA em uma sequencia especifica ("restrita") de reconhecimento de nucleotideos.
A development in software of endonuclease analysis makes PCR-RFLP much more convenient and only requires us to analyze the sequences in order to predict the digestion results.
5) The accuracy of endonuclease digests should be verified;
For example, Methanococcus jannaschii produces a specific restriction endonuclease found to be useful in cleavage of small single-stranded DNA "flaps.