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The main limitations of this technique are the decreased viability of the amoebal cells and their encystment at high incubation temperature, which do not allow the recovery of bacteria requiring a temperature [greater than or equal to] 37[degrees]C. Although incubation at 37[degrees]C may be ideal for bacterial recovery, lower temperatures (30[degrees]C-35[degrees]C) are generally used to prevent amoebal death or encystment (12,13,20). |
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