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electroporation |
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electroporation [-pôrā′shən] a type of osmotic transfection in which an electric current is used to produce temporary holes in cell membranes, allowing the entry of nucleic acids or macromolecules (a way of introducing new deoxyribonucleic acid into the cell). See also transfection. electroporation (i·lekˈ·trō·p n technique by which cell membranes are made permeable by rapid pulses of high-voltage current. Has been used to treat cancers. electroporation the use of high-voltage electrical impulse to create pores through a cell membrane and allow uptake of DNA into a cell. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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However, because it has been shown that i), free nickel molecules have a cytotoxic effect on cells when internalized endocytically (24); and ii), the cytotoxic effect of molecules normally internalized endocytically is amplified in electroporated cells (25), nickel toxicity will be a concern moving forward when examining long-term electroporative effects in cells. To determine what effect lung injury has on gene delivery, as opposed to gene expression, lungs of mice were injured with LPS and, 1 day later, luciferase-expressing plasmids were electroporated into the lungs and assayed for gene delivery and expression 1 and 2 days after electroporation (Figure 3). Virus recovered from baby hamster kidney cells electroporated with transcribed RNA was used for all experiments. |
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