electroporation

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e·lec·tro·por·a·tion

(ē-lek'trō-pōr-ā'shŭn),
A technique in which a brief electric shock is applied to cells; momentary holes open briefly in the plasma membrane, allowing the entry of macromolecules (for example, a way of introducing new DNA into a cell).

electroporation

(ĭ-lĕk′trō-pə-rā′shən)
n.
A process of applying a high-voltage electrical pulse to a living cell, causing temporary permeability of the cell membrane, through which a foreign material such as DNA may pass.

electroporation

[-pôrā′shən]
a type of osmotic transfection in which an electric current is used to produce temporary holes in cell membranes, allowing the entry of nucleic acids or macromolecules (a way of introducing new deoxyribonucleic acid into the cell). See also transfection.

electroporation

a method in which CELLS are subjected to an electrical impulse that leads to the temporary formation of pores in the cell MEMBRANE. Such pores enable commodities such as DNA, PROTEINS and ATP to pass into the cell, before the pores are repaired. Electroporation provides a means of transforming cells (see TRANSFORMATION). It can also be used to deliver DRUGS across the skin and to transfer GENETIC MATERIAL to targetted cells, in GENE THERAPY.

electroporation (i·lekˈ·trō·p·rāˑ·shn),

n technique by which cell membranes are made permeable by rapid pulses of high-voltage current. Has been used to treat cancers.

electroporation

the use of high-voltage electrical impulse to create pores through a cell membrane and allow uptake of DNA into a cell.
References in periodicals archive ?
Results demonstrated a linear correlation between the amount of CD40L RNA electroporated into the DCs and the number of DCs capable of secreting IL-12.
Four hundred microliters of the cell suspension were electroporated in a 4 mm cuvette using 10 knock-in vectors with four-1 ms pulses using 400 V capacitive discharges by BTX Electro-cell manipulator (ECM 2001, BTX, USA).
Stimulating this receptor signal cascade through ectopic expression of this novel CD40L RNA results in secretion of the downstream inflammatory cytokine IL-12 from the electroporated DCs.
Because cell survival is nearly guaranteed, a single cell can be electroporated multiple times, enabling experiments not previously possible.
Genetronics Biomedical Limited (Amex: GEB; Toronto) announced today that the journal "Human Gene Therapy" published the results of a pre-clinical study using Genetronics electroporation technology that showed an average five-hundred-fold increase in gene expression in electroporated muscle tissue compared with non-electroporated muscle.
Argos Therapeutics today announced the publication of a paper in the October 2008 issue of the Journal of Immunotherapy demonstrating that the Company's process of maturing dendritic cells (DC) electroporated with antigen-encoded messenger RNA (mRNA) is capable of driving long-term, stable expansion of cytolytic antigen-specific T cells that express effector/memory phenotype.
Much like "smart bombs", electroporated blood cells could be armed with drugs or genes that target sites in the body such as tumors, blood clots, and diseased organs.
Healey will highlight Argos' proprietary dendritic cell optimization process, Arcelis, which involves co-loading post-maturation electroporated (PME) dendritic cells with CD40L RNA and tumor antigen RNA.
She said the Company has patented every way it can prove that drugs or genes can be electroporated into the body.
Connie Schmaljohn of USAMRIID at Fort Detrick, MD, will present preclinical data from electroporated DNA vaccines for key biodefense targets; and Dr.
Results of preclinical experiments using the Genetronics proprietary electroporation catheter to deliver the cardiovascular agent heparin into rabbit arterial walls for restenosis prevention showed a significantly stronger heparin fluorescence throughout the vessel layers for at least 12 hours in the electroporation-treated tissue compared to the non- electroporated tissue.