electrophoresis


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Related to electrophoresis: Protein electrophoresis, Hemoglobin electrophoresis

electrophoresis

 [e-lek″tro-fo-re´sis]
the movement of charged particles suspended in a liquid on various media (e.g., paper, gel, liquid) under the influence of an applied electric field. adj., adj electrophoret´ic. The various charged particles of a particular substance migrate in a definite and characteristic direction—toward either the anode or the cathode—and at a characteristic speed. This principle has been widely used in the separation of proteins and is therefore valuable in the study of diseases in which the serum and plasma proteins are altered. The principle also has been applied in the separation and identification of various types of human hemoglobin.

e·lec·tro·pho·re·sis

(ē-lek'trō-fōr'ē-sis),
The movement of particles in an electric field toward an electric pole (anode or cathode); used to separate and purify biomolecules.
See also: electropherogram.
[electro- + G. phorēsis, a carrying]

electrophoresis

/elec·tro·pho·re·sis/ (e-lek″tro-fah-re´sis) the separation of ionic solutes based on differences in their rates of migration in an applied electric field. Support media include paper, starch, agarose gel, cellulose acetate, and polyacrylamide gel, and techniques include zone, disc (discontinuous), two-dimensional, and pulsed-field.electrophoret´ic
counter electrophoresis  counterimmunoelectrophoresis.

electrophoresis

(ĭ-lĕk′trō-fə-rē′sĭs)
n.
1. The migration of charged colloidal particles or molecules through a stationary medium under the influence of an applied electric field usually provided by immersed electrodes. Also called cataphoresis.
2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.

e·lec′tro·pho·ret′ic (-rĕt′ĭk) adj.

electrophoresis

[ilek′trōfərē′sis]
Etymology: Gk, elektron + pherein, to bear
the movement of charged suspended particles through a liquid medium in response to changes in an electric field. Charged particles of a given substance migrate in a predictable direction and at a characteristic speed. The pattern of migration can be recorded in bands on an electrophoretogram. The technique is widely used to separate and identify serum proteins and other substances. electrophoretic, adj.

electrophoresis

Lab methods A method of separating large molecules–eg, DNA fragments or proteins from a mixture of similar molecules, by passing an electric current through a medium containing the mixture; each molecule travels through the medium at a different rate, depending on its electrical charge and size; agarose and acrylamide gels are media commonly used electrophoretic media

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
See also: electropherogram
Synonym(s): ionophoresis, phoresis (1) .

electrophoresis

Separation of charged particles in a solution (ions) by the application of an electric current. This can be done in a thin layer of solution on paper or in a gel. Ions of low weight move more quickly than those of high weight, so separation occurs and can be demonstrated by staining. The method is widely used in medicine to identify and measure the proteins present in the blood including the ANTIBODIES (IMMUNOGLOBULINS). It is used to identify the various abnormal haemoglobins causing SICKLE CELL ANAEMIA and other similar conditions. It is extensively used in genetic work such as DNA fingerprinting. Electrophoresis is remarkably sensitive. Pieces of DNA, for instance, that differ in length from each other by only one base pair can be separated into discrete bands by this method.

electrophoresis

a method for separating particles with different electrical charges, for example, amino acids, peptides, proteins and nucleic acids. The apparatus consists of a supporting medium soaked in a suitable buffer with an electrical field set up across it. The mixture to be separated (e.g. blood proteins) is placed on the supporting medium. The components with different charges then separate from each other and their eventual position is compared with the position of known standards.

Electrophoresis

Use of an electrical field to separate proteins in a mixture (such as blood or urine), on the basis of the size and electrical charge of the proteins.

electrophoresis

movement of particles in an electric field, towards the anode or cathode

electrophoresis (i·lekˈ·trō·f·rēˑ·sis),

n method used to separate particles, such as DNA or proteins, in which an electric current is passed through the medium and the separation of the molecules depends on the rate at which they travel towards the electrode based on their electrical charge.

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
Synonym(s): ionophoresis, phoresis (1) .

electrophoresis,

n the movement of charged suspended particles through a liquid medium in response to changes in an electric field.

electrophoresis

the movement of charged particles suspended in a liquid through various media, e.g. paper, cellulose acetate, gel, liquid, under the influence of an applied electric field.
The various charged particles of a particular substance migrate in a definite and characteristic direction—toward either the anode or the cathode—and at a characteristic speed. This principle has been widely used in the separation of proteins and nucleic acids and is therefore valuable in the study of diseases in which the serum and plasma proteins are altered. See also immunoelectrophoresis.

SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
a procedure that revolutionized the analysis of complex mixtures of proteins. The proteins are solubilized by the powerful, negatively charged detergent sodium dodecyl sulfate (SDS) which causes proteins to unfold into extended, single polypeptide chains. A reducing agent such as mercaptoethanol is usually added to break disulfide bonds. The constituent polypeptides are then electrophoresed through an inert matrix of highly cross-linked gel of polyacrylamide. The pore size of the gel can be varied by altering the concentration of polyacrylamide.
two-dimensional gel electrophoresis
a SDS-polyacrylamide gel electrophoresis run, first in one direction, then again at right angles. In the first dimension an isoelectric-focusing gel is run and in the second dimension the proteins are separated in SDS-PAGE. A greater number of individually different proteins can be resolved in a highly repeatable fingerprint-like pattern.
References in periodicals archive ?
The Asia-Pacific region is expected to be the fastest-growing electrophoresis technologies market during the forecast period, primarily due to the increasing investments by pharmaceutical and biotechnology companies, rising demand of innovative technologies, and increasing research collaborations and funding.
The market of protein diagnostics, one of the major applications of electrophoresis, is expected to grow at a highest CAGR of 5.
High-resolution separations based on electrophoresis and electroosmosis.
An area where capillary electrophoresis has shown excellent potential, as a separations technique, is in the analysis of isomers.
Kohn presented his second paper on CA electrophoresis at a protein conference in Bruges, Belgium, in May 1957 (14).
Pediococcus, Leuconostoc and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.
2]; exposure times, 24 and 48 hr) and cisplatin-treated DNA (concentration, 100 [micro]M; exposure time, 24 hr) after DNA agarose gel electrophoresis.
With the addition of Lab901's outstanding technology and talented team, Agilent can now address customer needs across the entire span of electrophoresis life science applications - from semi-automated to 96-well-plate compatible workflows," said Patrick Kaltenbach, vice president of Agilent's Liquid Phase Separations business.
1) described a case in which the Sebia semiautomated electrophoresis method failed to demonstrate proteinuria in a 50-fold concentrated urine sample despite the presence of >2 g/24 h total protein.
In this study, we compared pulsed-field gel electrophoresis patterns of Aeromonas isolates from stool specimens of patients with diarrhea with Aeromonas isolates from patients' drinking water.
We observed that DMSO and CAT basically abolished most of the As-induced DNA single strand breaks (SSBs) in human cells with single-cell gel electrophoresis (SCGE) assay (5).
A standard technique for separating DNA pieces of different sizes, electrophoresis relies on an electric field to gently draw charged DNA particles of varying sizes down a gel.
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