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Three DE-genes are also found in our data: NADK (NAD kinase), LOC422993 (Similar to interferon-induced membrane protein Leu-13/9-27), and LAMP3 (lysosomal-associated membrane protein 3) (Figure 4), suggesting potential crucial function of these genes in not only early stage of eggshell calcification but also other stages of eggshell formation.
These three genes, FSTL1 (follistatin-like 1), CAMK2D (calcium/ calmodulin-dependent protein kinase (CaM kinase) II delta) and KRT75 (keratin 75) (Figure 4), could reflect potential interaction of eggshell calcification and organic matrix formation.
Eggshell calcification requires considerable ion transportation, especially [Ca.