A histopathologic examination revealed a well-differentiated SCC with an inverted and warty growth pattern, keratinization with keratin pearl and dyskeratotic
2) G III (3) inflammation G IV (1) Groups Dermatological (n) BMT+GVHD+MSC G I: Focal/diffuse vacuolization of basal-layer (1) G II: Subepidermal infiltration of lymphocytes (1) CsA+MTX G I: Focal/diffuse vacuolization of basal layer (3) MSC G I: Focal/diffuse vacuolization of basal layer (3) CsA+MTX+MSC G I: Focal/diffuse vacuolization of basal layer (2) BMT-CONTROL G I: Focal/diffuse vacuolization of basal-layer (4) G II: Spongiose+ dyskeratotic
Keratinocyte lymphocytes infiltration (3) G III: Subepidermal separation (1)
The epithelium has dyskeratotic
cells with markedly atypical, often pleomorphic nuclei.
The surface part of the squamous papilloma with prominent nuclear dysplasia and the loss of stratification and polarisation in hypercellular squamous epithelium were seen and also the papilloma showed prominent dysplastic figures and dyskeratotic
Stratum corneum thickness and the degree of epidermal dyskeratosis were evaluated by using a four-point scoring system: 0, none; 1, mild; 2, moderate; and 3, severely dyskeratotic
or thickened stratum corneum.
Histologically, both Hailey-Hailey disease and Darier's disease have similar features (acantholytic suprabasal keratinocytes); however, Darier's disease also has dyskeratotic
Suprabasilar clefts with acantholytic and dyskeratotic
cells were located adjacent to and extending into the lumen of the cystic cavity; orthokeratosis and parakeratosis were present (Fig.
Also associated with UV irradiation of human skin is the appearance of dyskeratotic
keratinocytes, known as sunburn cells, in the superficial layers of the epidermis.
In grade I, there is vacuolization of the basal keratinocytes (Figure 1); in grade II, there are dyskeratotic
keratinocytes and basal cell vacuolization; in grade III, there is increased keratinocyte necrosis and focal basal layer clefting; and in grade IV (Figure 2), there is necrosis of the entire epidermis and complete separation from the dermis.
A punch biopsy revealed a slightly thinned epidermis with abundant dyskeratotic
cells and vacuolar change along the basal layer.
The Darier-like pattern consists of suprabasal acantholysis of keratinocytes with scattered apoptotic or dyskeratotic
cells within various levels of the epidermis (Figure 2).
In contrast to common dyskeratotic
cells, which have a dense eosinophilic cytoplasm and mature to parakeratotic cells, pagetoid dyskeratotic
cells are pale, show intercellular prickles with the adjacent keratinocytes, and mature to orthokeratotic keratinization.