Crowle's double stain
is prepared in developer and rinse solutions.
Even though RPp40 antibodies are said to have greater specificity in lung cancers, our experience with 2 commercially available RPp40 antibodies has demonstrated varying degrees of cytoplasmic background staining, and thus, they may not be suitable for use in multiplex stains, such as double stains
for prostate cancer.
The slides were incubated with primary antibody for 30 minutes followed by double staining detection with MACH 2 double stain
2 for 30 minutes and developed with diaminobenzidine brown chromogen for TTF-1 and DG3 + CK5 and warp red for p40 and napsin A.
Digital image analyses of a Ki67/MART1 double stain
and a mimicked Ki67 single stain are shown in Figure 1.
To determine if a double stain
for MUC/p53 improved specificity and sensitivity for distinction of pancreatic andenocarcinoma from chronic pancreatitis by comparing maspin, mucin 4 (MUC4), p53, Smad4, and the double stain
For double stains
, both primary antibodies were added together, as were secondary antibodies since the two primary antibodies had different host species (rabbit for FMRFamide and mouse for tubulin).
The double stains
were performed on the slides that were previously stained with pan-CK or CAM5.