ping-pong mechanism

(redirected from double displacement mechanism)

ping-pong mech·a·nism

a special multisubstrate reaction in which, for a two-substrate, two-product (that is, bi-bi) system, an enzyme reacts with one substrate to form a product and a modified enzyme, the latter then reacting with a second substrate to form a second, final product, and regenerating the original enzyme. An example of such a mechanism is found in the aminotransferases. More complex ping-pong mechanisms exist for enzymes having more than two substrates.
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These mutants of retaining glycosidases have had the catalytic glutamate nucleophile replaced with alanine, glycine, or serine, preventing the usual double displacement mechanism for glycosidic bond cleavage.