direct antiglobulin test

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Related to direct antiglobulin test: Coombs test, Indirect Antiglobulin Test

direct antiglobulin test

Direct Coombs test Immunology A test to detect immune hemolysis caused by binding of Ig and/or complement components to RBCs after sensitization to an antigen–eg Rh factor–on the RBC surface; a DAT helps differentiate autoimmune and 2º immune hemolytic anemia, which can be drug-induced or associated with underlying disease such as lymphoma. See Indirect antiglobulin test.

direct antiglobulin test

Abbreviation: DAT
A laboratory test for the presence of complement or an antibody that is bound to a patient's red blood cells (RBCs). The test is used in patients with autoimmune hemolytic anemia, hemolytic disease of the newborn, and transfusion reactions. After the patient's RBCs are washed to remove unbound antibodies, they are mixed with antihuman globulin serum containing polyvalent antibodies that bind with the antibody or complement on the RBCs and cause them to agglutinate (clump). Monoclonal antibodies can be used to identify the specific class of antibody or complement component causing RBC destruction. See: Coombs' test


without intervening steps.

direct antiglobulin test
see direct coombs test.
direct capture ELISA mastitis test
measures polymorphonuclear antigens in milk sample as an indication of elevated milk cell counts and therefore presence of mastitis.
direct contact
see direct contact.
direct costs
see variable costs.
direct effect
the effect of one variable on another without passing through a third variable.
direct immunofluorescence testing
see fluorescence microscopy.
direct oxidative pathway
see pentose phosphate pathway.
direct relationship
see direct relationship.
References in periodicals archive ?
Positive direct antiglobulin test results after intravenous immune globulin administration.
An explanation of the failure of the direct antiglobulin test to detect erythrocyte sensitization in ABO haemolytic disease of the newborn and observations on pinocytosis of IgG anti-a antibodies in infant (cord) red cells.
False-negative direct antiglobulin tests are usually due to low-affinity autoantibodies that spontaneously elute from the red cell in vitro or to amounts of erythrocyte-coating antibodies that are below the limit of detection by the antiglobulin test.
Concurrent AIHA cannot be excluded, especially in view of the positive IgG result in the direct antiglobulin test, and may have contributed to the severity of the patient's hemolytic anemia.
A direct antiglobulin test is done on the posttransfusion red cells.
For example, whether there's a positive direct antiglobulin test or an incompatible crossmatch, we call the clinician, notify him of the problem, document the call, and transcribe the results on a special antibody sheet.
Bio-Rad provides a wide variety of platforms, reagents, data management, and connectivity solutions to address different blood typing needs, offering efficient and reliable results for blood grouping, phenotyping, crossmatching, antibody screening and identification, direct antiglobulin tests, and single antigen typing.
ATLANTA -- Healthy blood donors with positive direct antiglobulin tests develop cancer more than twice as often as those who test negative, according to a provocative study that linked thousands of blood bank records to Israel's national cancer registry.

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