direct agglutination test


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direct agglutination test

a test for the presence of antibodies to a specific antigen in which a dilute antiserum is mixed with the antigen in question.
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Reagents and card / cassette in amounts calculated on the amount of performed tests - Blood group (ABO and Rh system, immune antibodies in the test PTA) - 45 000 examinations for 3 years,- Newborn blood group (ABO and Rh system - 2serie, polyvalent serum BTA - 2 100 tests for 3 years,- Blood recipients blood group (ABO and Rh system, immune antibodies in the test PTA - 30 600 tests for 3 years,- Blood group donors and compatibility tests (checking the ABO and Rh group donor, proper attempt conformity) - 53 500 tests for 3 years,- Identification of antigen DVI direct agglutination test in newborns - 600 tests for 3 years.
Nongovermnent healthcare providers and Government of Southern Sudan--administered healthcare facilities thus had to confirm suspected kala-azar by direct agglutination test before they could receive a supply of the first-line treatment--sodium stibogluconate--from WHO through Pharmaciens sans Frontieres.
The AUW Biomedical Research Laboratory (ABRL), as the WHO and Federal Ministry of Health focal institution, has produced two batches of Direct Agglutination Test (DAT) Antigen used for diagnosis of Kala-azar in Sudan.
From January 2000 to February 2006, a total of 3,645 patients suspected of having kala-azar were screened at Amudat Health Centre by using the direct agglutination test or rK39 antigen-based dipsticks (6); 2,088 patients with confirmed disease were treated with daily intramuscular injections of sodium stibogluconate or meglumine antimonite, 20 mg/kg bodyweight, for 30 days.
It was not possible to predict the class of antibodies by using direct agglutination tests.

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