dihydrofolate reductase


Also found in: Acronyms, Wikipedia.

di·hy·dro·fo·late re·duc·tase (DHFR),

(dī-hī'drō-fō'lāt rē-dŭk'tās), [MIM*126060]
An enzyme reversibly oxidizing tetrahydrofolate to 7,8-dihydrofolate with NADP+. A crucial enzyme in one-carbon metabolism; used as a marker of drug resistance to methotrexate.
Synonym(s): 5, 6, 7, 8-tetrahydrofolate dehydrogenase

dihydrofolate reductase

An enzyme necessary for the synthesis of the purines of DNA.

dihydrofolate reductase

enzyme catalyzing the conversion of folate to 5,6,7,8-tetrahydrofolate, which is the key carrier of one-carbon units in purine and pyridime synthesis, the pathway for the breakdown of histidine and the synthesis of S-adenosylmethionine from S-adenosylhomocysteine. This enzyme is the target for methotrexate, which inhibits enzyme action, a key means of inducing remissions in acute leukemias.
References in periodicals archive ?
The basic mechanism of pyrimethamine against malaria is to target on folic acid which produces tetrahydrofolates by inhibiting the enzyme dihydrofolate reductase (DHFR) (Yuvaniyama et al.
Key determinants in the occurrence of clonal variation in humanized antibody expression of cho cells during dihydrofolate reductase mediated gene amplification.
Dihydrofolate reductase enzyme inhibition assay for plasma methotrexate determination using a 96-well microplate reader.
Structure of Plasmodium vivax dihydrofolate reductase determined by homology modeling and molecular dynamics refinement.
chloroquine and quinine); (ii) antifolates, which are dihydrofolate reductase (DHFR) (e.
The reduction of dihydrofolate to THF is mediated by a bifunctional dihydrofolate reductase that also has thymidylate synthase activity.
Splicing together dihydrofolate reductase (DHFR) enzymes from Bacillus subtilis (B.
The design, implementation and broad applications of this strategy are illustrated with a large number of enzymes with particular detail provided for the example of murine dihydrofolate reductase (DHFR).
One group of such agents is dihydrofolate reductase inhibitors, which block the conversion of folate to its more active metabolites.
19] These targets include genes that code for dihydrofolate reductase, thymidine kinase, DNA polymerase-[Beta], thymidylate synthetase, B-Myb, cdc2, cyclin A, cyclin E, and c-Myc; they also include the E2F proteins themselves.
This vector contains a human [beta]-globin gene whose expression is controlled by elements of the globin hypersensitive region, and a mutant form of dihydrofolate reductase (DHFR) for use as a selectable marker.
More important, the easily understandable rules provided insights directly comparable to the relevant literature concerning the known shape of pockets in the binding site of dihydrofolate reductase; inhibition of dihydrofolate reductase is the mechanism used by the antifolate drugs methotrexate and trimethoprin for treating a range of diseases, including many cancers, infections, rheumatoid arthritis, multiple sclerosis, and lupus.