digoxigenin


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di·gox·i·gen·in

(dī-joks'ĭ-jen-in),
The aglycon of digoxin that is joined by 3 moles of digitoxose to form the glycoside, digoxin.

digoxigenin

/di·goxi·ge·nin/ (dĭ-jok″sĭ-je´nin) the steroid nucleus that is the aglycone of digoxin.
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Tissue-specific cPdcl2 expression was examined in the brain, liver, muscle, spleen, kidney, testis, and ovary of 24-week-old chickens by Northern blot using a digoxigenin Northern Starter Kit (Roche; Holtke et al.
When we performed a quantitative comparison between the PNA-ISH and DNA-ISH assays, using the same batch of 1319-infected UT-7/EpoS1 cells in 5 different experiments, a mean of 21 of 100 counted cells were positive by the biotinylated PNA probe vs a mean of 10 of 100 cells with the digoxigenin DNA probe.
The endogenous biotin-like reactivity prompted us to use a digoxigenin detection system.
For the preparation of sense and antisense Digoxigenin (DIG)-labeled RNA probes, total RNA was isolated from chicken embryos using TRIzol reagent (Invitrogen, Shanghai, China).
Amplified plasma DNA and reference DNA were labeled with biotin-16-dUTP and digoxigenin (dig)-11-dUTP (Boehringer-Mannheim), respectively, by nick translation.
Custom probes were synthesized with the incorporation of digoxigenin at the 5' end (Operon Biotechnologies Inc.
Immunohistochemical detection of digoxigenin: We used a DIG nucleic acid detection kit (Boehringer Mannheim, Indianapolis, TN) to detect the digoxigenin tagged in situ RT-PCR amplified products.
After hybridization, the slides were incubated with fluorescein isothiocyanate (FITC)-conjugated antibodies (Boehringer Mannheim) to digoxigenin, and Texas Red-conjugated antibodies (Vector Laboratories, Inc, Burlingame, Calif) to biotin, which produce green and red signals, respectively.
The probe was produced by direct digoxigenin (DIG)-labeled PCR by using the PCR DIG probe synthesis kit (Roche Diagnostics, Basel, Switzerland) and the primers Kleb_MP_F1 (5'-GAG CAA AGT TAC TGT TTC TAT GGA-Y) and Kleb_MP-R1 (5'-TGA GCC ATC TTT CAT CAA CC-3') on the K.
The resulting probe Perksp700DIG (5'-CGCACAGTTAAGTRCGTGRGCACG-3') was 5' end-labeled with digoxigenin (Sigma-Genosys, The Woodlands.
01%) digoxin or its aglycone, digoxigenin, and only slightly detected dihydrodigoxin (<0.
In this assay, PCR products are labeled with both digoxigenin (DIG) and biotin during amplification by means of labeled oligonucleotides.