digoxigenin


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di·gox·i·gen·in

(dī-joks'ĭ-jen-in),
The aglycon of digoxin that is joined by 3 moles of digitoxose to form the glycoside, digoxin.

digoxigenin

/di·goxi·ge·nin/ (dĭ-jok″sĭ-je´nin) the steroid nucleus that is the aglycone of digoxin.
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The PCR solution and carbon particles conjugated with avidin were directly added to the nitrocellulose membrane with two test lines of immobilized antibodies specific for digoxigenin and fluorescein.
For labeling of PCR products, the reaction of PCR was performed by dNTP mixture containing digoxigenin labeling mix (Digoxigenin dNTPs, Roche, Germany) with the same condition.
The synthesis of the digoxigenin labeled riboprobe and the hybridization reactions with the polyprobe were conducted as previously described (HERRANZ et al.
Some of the useful plant drugs include vinblastine, vincristine, Taxol, Podophyllotoxin, camptothecin, digitoxigenin, gitoxigenin, digoxigenin, tubocurarine, morphine, codeine, aspirin, atropine, Pilocarpine, capsicum, Ellison, curcumin, artemesinin and ephedrine among others.
After hybridization, the membranes were washed at high stringency, bound probes were detected using phosphatase-conjugated antibody to digoxigenin (Roche Applied Science), and fluorescence was captured by an imaging system (Storm[TM] 860, GE Healthcare Life Science).
for patient 2 painting probes for chromosomes 15 and 22 were digoxigenin labeled (WCP 15 and WCP 22; Oncor, Gaithersburg, MD, USA); for patient 4 painting probes for chromosomes 1, 9, and 22 (WCP 1, 9, and 22; XCP-MetaSystems); for patient 6 painting probes for chromosomes 4, 8, 9, and 22 (WCP 4, 8, 9, and 22; XCP-MetaSystems and Cytocell).
For detection of biotin and digoxigenin, the samples were incubated with Cy3.
The Digoxigenin (DIG) system for non radioactive labelling and detection of nucleic acids- an overview.
To verify the introgression of perennial teosinte chromosome segments into MZI202, maize genomic DNA was digoxigenin labeled and its signals were detected with FITC.
Both antisense and sense (used for controls) gene-specific oligonucleotides were synthesized, labeled with digoxigenin (DIG)-dUTP, and used as probes.
2 [[micro]gram]) were labeled by the digoxigenin nonradioactive labeling method (Boehringer Mannheim Co.
The probe - an RNA mirror image of apple scar skin viroid developed in the National Germplasm Resources lab by Hadidi - is labeled along its length with digoxigenin (DIG).