digitonin


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dig·i·to·nin

(dij'i-tō'nin),
1. A steroid glycoside obtained from Digitalis purpurea that has no cardiac action; used as a reagent in the determination of plasma cholesterol and steroids having a 3-hydroxyl group in beta configuration.
2. A mixture of four different steroids found in the seeds of Digitalis purpurea; a strong hemolytic poison. They can act as nonionic detergents in the solubilization of membrane proteins.
Synonym(s): digitin

digitonin

/dig·i·to·nin/ (dij″ĭ-to´nin) a saponin from Digitalis purpurea with no cardiotonic action; used as a reagent to precipitate cholesterol.

digitonin

a saponin in Digitalis purpurea. It has no effect on heart muscle.
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References in periodicals archive ?
Osteosarcoma cybrids were permeabilized with digitonin (4 [micro]M), and respiration was initiated with complex I substrate (malate/pyruvate, 5 mM).
A problem of using digitonin as a solubilizing agent is that it exists as large micelles, which makes it difficult to characterize solubilized muscarinic receptors in terms of molecular mass: the ligand binding activity in a digitonin extract was found in the fraction corresponding to a mass of around a million.
6 [micro]mol/L digitonin for the permeabilization of the cell membranes, 1 mmol/L pyruvate, 1 mmol/L malate, and, where indicated, 2.
dodecyldimethylamine oxide, lysolecithins), and ii) detergents belonging to the group of bile salts or other steroid-based detergents such as 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfon ate (CHAPS) and digitonin.
6]) were washed with ice-cold Dulbecco's phosphate-buffered saline and were resuspended in 100 [micro]L of ice-cold digitonin lysis buffer (0.
1% digitonin and plating on trypticase soy agar (TSA).
Germinally transgenic tadpoles were produced by restriction enzyme-mediated integration nuclear transplantation according to Kroll and Amaya (1996), with the following modifications: sperm was purified by centrifugation on a two-layer discontinuous Percoll (Sigma) gradient before the permeabilization step, which was performed with digitonin (Sigma) instead of lysolecithin.
The muscarinic acetylcholine receptors (mAChR), which are members of the family of receptors that are coupled to guanine nucleotide binding proteins (G proteins) and whose structure is characterized by the seven membrane-spanning [alpha]-helical domains [2, 3], are usually solubilized in digitonin or in a mixture of digitonin and sodium cholate, which have yielded more than 50% of the soluble receptors with retention of ligand binding activity [4, 5].