digitonin


Also found in: Dictionary, Encyclopedia, Wikipedia.

dig·i·to·nin

(dij'i-tō'nin),
1. A steroid glycoside obtained from Digitalis purpurea that has no cardiac action; used as a reagent in the determination of plasma cholesterol and steroids having a 3-hydroxyl group in beta configuration.
2. A mixture of four different steroids found in the seeds of Digitalis purpurea; a strong hemolytic poison. They can act as nonionic detergents in the solubilization of membrane proteins.
Synonym(s): digitin

digitonin

/dig·i·to·nin/ (dij″ĭ-to´nin) a saponin from Digitalis purpurea with no cardiotonic action; used as a reagent to precipitate cholesterol.

digitonin

a saponin in Digitalis purpurea. It has no effect on heart muscle.
Mentioned in ?
References in periodicals archive ?
6]) were washed with ice-cold Dulbecco's phosphate-buffered saline and were resuspended in 100 [micro]L of ice-cold digitonin lysis buffer (0.
Behavior of polymer supported digitonin with cholesterol in the absence and presence of butter oil.
These vacuoles were positive for Sudan III, Sudan black B, oil red O, Nile blue, periodic acid-Schiff, Schultz test, and digitonin stain, and were negative for performaric acid--Schiff stains.
6 [micro]mol/L digitonin for the permeabilization of the cell membranes, 1 mmol/L pyruvate, 1 mmol/L malate, and, where indicated, 2.
Germinally transgenic tadpoles were produced by restriction enzyme-mediated integration nuclear transplantation according to Kroll and Amaya (1996), with the following modifications: sperm was purified by centrifugation on a two-layer discontinuous Percoll (Sigma) gradient before the permeabilization step, which was performed with digitonin (Sigma) instead of lysolecithin.
Additionally, in threedrug combinations of lignans with the saponin digitonin and doxorubicin MDR reversal activity was even stronger enhanced.
The muscarinic acetylcholine receptors (mAChR), which are members of the family of receptors that are coupled to guanine nucleotide binding proteins (G proteins) and whose structure is characterized by the seven membrane-spanning [alpha]-helical domains [2, 3], are usually solubilized in digitonin or in a mixture of digitonin and sodium cholate, which have yielded more than 50% of the soluble receptors with retention of ligand binding activity [4, 5].
The results were expressed as the percentage of ATP released relative to the total ATP present in cells lysed by means of digitonin (50 [micro]M) (16).