differential display


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differential display

the use of RT-PCR-based technologies to amplify mRNA from specific cells or tissues and then to compare them directly with amplified mRNA from another cell or tissue.

dif·fer·en·tial dis·play

(dif'ĕr-en'shăl dis-plā')
Use of reverse transcriptase and polymerase chain reaction technologies to amplify mRNA from specific cells or tissues and then to compare them directly with amplified mRNA from another cell or tissue.

differential display

,

DD

A gene-screening technique used to analyze the ways in which different types of messenger RNA are expressed in organisms.
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To study its possible mechanism in response to the molecular machinery in the antlers between the male and female reindeer, we decided to perform a preliminary study to examine differences in the gene expression in relationship to antler mesenchyme of both sexes using the mRNA differential display method.
Identification of sexually dimorphic gene expression in brain tissue of the fish Leporinus macrocephalus through mRNA differential display and real time PCR analyses.
Using the differential display, Ansari and Lin (2011) cloned several genes for dehydration in S.
Three new osmotic stress-regulated cDNAs identified by differential display polymerase chain reaction in rapeseed leaf discs.
The differential display method was used to perform a comparative gene analysis on cultured B.
The authors used a differential display method of bladder cancer vs healthy urothelial tissue and mass spectrometry to identify proteins that are increased in cancer tissue.
Application of modified differential display technique for cloning and sequencing of the 3' region from three putative members of wheat HSP70 gene family.
Differential Display method facilitates for up and down regulation, presence and absence of fragments, qualitative and quantitative difference and signals of varying intensity [25].
assemble 20 chapters on recent research and applications of differential display technology in gene-hunting.
Alternative genomic technologies, such as differential display and serial analysis of gene expression (SAGE) are mentioned but not described.
Differential display analysis (DDA) has become a useful and popular technique to identify differentially expressed genes in many tissues and cell types.
The genes had been previously identified by differential display reverse transcriptase-polymerase chain reaction, a method that screens thousands of RNA messages to identify genes that are turned on or off by specific treatments.

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