differential display


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differential display

the use of RT-PCR-based technologies to amplify mRNA from specific cells or tissues and then to compare them directly with amplified mRNA from another cell or tissue.

dif·fer·en·tial dis·play

(dif'ĕr-en'shăl dis-plā')
Use of reverse transcriptase and polymerase chain reaction technologies to amplify mRNA from specific cells or tissues and then to compare them directly with amplified mRNA from another cell or tissue.

differential display

,

DD

A gene-screening technique used to analyze the ways in which different types of messenger RNA are expressed in organisms.
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We chose a composite selection of seven candidate genes isolated by differential display from both tadpole and metamorph brains for the multiple-gene dot blot.
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Study of mRNA expression of Arabidopsis thaliana under stimulation using modified differential display RT-PCR with silver staining, Colloids Surfaces B: Biointerfaces, 40: 31-34.
The authors used a differential display method of bladder cancer vs healthy urothelial tissue and mass spectrometry to identify proteins that are increased in cancer tissue.
The PGI discovery program begins with the creation of a proprietary database of normal, diseased and drug-associated gene expression patterns using cutting-edge differential display and gene expression monitoring technology.
These services include genomic data management, microarray support, quantitative PCR, differential display, genotyping, genetically-modified organism testing, and a battery of other molecular biology services which support researchers in the pharmaceutical, biotechnology and academic sectors in their attempt to understand the mechanisms of disease and to discover and develop new drugs.
assemble 20 chapters on recent research and applications of differential display technology in gene-hunting.
Alternative genomic technologies, such as differential display and serial analysis of gene expression (SAGE) are mentioned but not described.
Differential display analysis (DDA) has become a useful and popular technique to identify differentially expressed genes in many tissues and cell types.

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