dideoxynucleotide


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dideoxynucleotide

a synthetic molecule used in enzymatic DNA sequencing.
References in periodicals archive ?
1 Cycle Sequencing Kit (Applied Biosystems, USA), comprising DNA polymerase, a mixture of deoxyribonucleotide triphosphates, and fluorochrome labeled dideoxynucleotide triphosphate.
Sanger Sequencing is known as the chain termination method or the dideoxynucleotide method or the sequencing by synthesis method.
Cycle sequencing reactions were performed at the Genomics Core Facility, Huck Institute for the Life Sciences, Penn State University, using 3' Big-Dye-labeled dideoxynucleotide triphosphates (v 3.
Gene Sequencing and Analysis: The purified amplicons were sequenced using fluorescent dideoxynucleotide terminators in an ABI PRISM 3130 automated sequencer (Applied Biosystem, Inc, Foster City, CA).
After hybridization, the primer is extended by the polymerase with dideoxynucleotide terminators in the absence of dNTPs.
5 [micro]M dideoxynucleotide triphosphate (dNTP) mix, and 10U RNase inhibitor (Invitrogen) in a volume of 20 [micro]L at 37[degrees]C for 1h.
Genomes were finished by using PCR and traditional dideoxynucleotide sequencing (Sanger) to resolve gaps and repetitive and low coverage areas.
In our experience pyrosequencing is cost-effective in testing samples when compared to qPCR, standard PCR, and dideoxynucleotide chain-terminating sequencing methods.
This partial actin gene fragment was sequenced by the dideoxynucleotide method from Centre for Applied Molecular Biology (CAMB), Lahore, Pakistan.
DNA sequencing employs poisonous dideoxynucleotide tri-phosphates (diNTPs) in a 1:1 ratio with the nonpoisonous dNTPs.
HIV RNA extraction, reverse transcription, amplification, dideoxynucleotide sequencing, and algorithmic interpretation of the sequencing data are steps of: