The PC12 cell as a model for studies of the mechanism of inducing of peripheral neuropathy by anti-HIV-l dideoxynucleoside
Assay-specific deoxynucleoside triphosphates (dATP, dGTP, dTTP; Amersham) and dideoxynucleoside
triphosphates (ddA, ddC, ddG, ddT; BioLog Life Science Institute) were added at 0.
Therefore, in that study, a very short extension strategy using alternating deoxynucleoside triphosphates (dNTPs) and dideoxynucleoside triphosphates was adopted so that the products differed in mass by [M.
75 [micro]M primer, 25 [micro]M each of the dideoxynucleoside triphosphates (Amersham Pharmacia Biotech), and 0.
For example, the new cut off values for the dideoxynucleoside
analogues, including d4T, are lower than before and, in a study of 5,000 random clinical samples, revealed a higher and more realistic incidence of resistance.
Our strategy for detecting the splice variants expressed from the genes is based on the extension of gene-specific minisequencing primers with fluorescent dideoxynucleoside triphosphates (ddNTPs), each of which define a specific splice variant (see Table 52 in the online Data Supplement).
1) Nonstandard abbreviations: HAEC, human aortic endothelial cell; HUVEC, human umbIIical vein endothelial cell; dNTP, deoxynucleoside triphosphate; ddNTP, dideoxynucleoside triphosphate; Ct, threshold cycle; s/n, signal-to-noise rafio; and s/i, signal iracfion.
However, treatment of HIV patients with dideoxynucleoside
(antiretroviral) therapy is recognized as a cause of painful neuropathy, which often persists long after the discontinuation of the therapy.
DNA polymerase extends it by adding one fluorescently labeled dideoxynucleoside
triphosphate complementary to the variant base.
Comparison of mitochondrial morphology, mitochondrial DNA content, and cell viability in cultured cells treated with three anti-human immunodeficiency virus dideoxynucleosides
This served as rationale for the synthesis of all 2',3'- dideoxynucleosides