diaminobenzidine


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diaminobenzidine

(dī″ă-mēn″ō-bĕn′zĭ-dēn″),

DAB

A chromogen, C12H14N4, used in immunocytochemistry to highlight peroxidase.
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Since MART1 is a cytoplasmic marker and Ki-67 is a nuclear marker, by using 2 different systems (eg, ABC and alkaline phosphatase) with 2 different chromogens (eg, diaminobenzidine [brown] and aminoethylcarbazole [red]), it is relatively easy to identify which cells express both markers, thus representing the fraction of melanocytes that are proliferating.
Biotinylated antibody, and 3-3' diaminobenzidine were obtained from Dako (Glostrup, Denmark).
The slides were washed as above and developed with diaminobenzidine (Dako).
Mouse monoclonal antibody clone 124 against bcl-2, rabbit anti-mouse IgG alkaline phosphatase conjugate, biotinylated rabbit anti-mouse antibody, the avidin-biotin-peroxidase complex kit, and diaminobenzidine were purchased from Dako.
After the filter was washed extensively, the apo(a) banding pattern was visualized through chemical staining for peroxidase with diaminobenzidine.
on 3-[micro]m-thick, formalin-fixed, paraffin-embedded sections using a mouse monoclonal antibody against p63 (clones 4A4 and Y4A3, dilution 1:200, Thermo Scientific, Fremont, California), antigen retrieval with tris(hydroxymethyl)aminomethane-based buffer (CC1, Ventana Medical Systems, Tucson, Arizona) and a diaminobenzidine detection kit (Ultra-View, Ventana Medical).
Arlington Heights, IL, USA) by using diaminobenzidine as the substrate for color development.
2 g/L diaminobenzidine (Serva, Heidelberg, Germany) in 1 mol/L Tris-buffered saline (pH 7.