Urea was measured by the direct diacetylmonoxime method modified by Mulvenna and Savidge (1992).
A modified manual method for the determination of urea in seawater using diacetylmonoxime reagent.
An improvement in determination of urea usng diacetylmonoxime
method with and without deproteinization.
A colorimetric assay based on the reactivity of carbamoyl groups with diacetylmonoxime
may be used (22).
Estimation of serum Urea: Urea reacts with hot acidic Diacetylmonoxime
in the presence of Thiosemicarbazide and produces a rose-purple coloured complex, which is determined colorimetrically .
Total protein was determined by Biuret method using Total Protein Kit from Crest Biosystems (Doumas, 1975), Albumin by BCG method using Albumin Kit from Crest Biosystems (Doumas, 1971) and Urea by Diacetylmonoxime
(DAM) method using Urea Kit from Span Diagnostics ltd (Wybenga, 1971).
In this procedure, diacetylmonoxime
, thiosemicarbazide, and the acid reagent were mixed on-line and combined with the sample carrier prior to the heating bath (95[degrees]C) and detector.
Urea concentration in urine or serum was measured using the diacetylmonoxime
method of Marsh et al.