Cover specimen and control smears with acid-alcohol destaining
Purified Protease has the commercial application of dehairing of goat skin, destaining
of blood stains and decomposing of gelatinous coating on the X-ray films which is mainly useful in recovery of silver.
DNA was visualized by UV after staining in an ethidium bromide solution for 20 min and destaining
in double-distilled [H.
in 500 mL/L acetonitrile-500 mL/L 50 mmol/L ammonium bicarbonate and dehydration in acetonitrile, bands were dried in a SpeedVac.
We used a destaining
solution of 45% ethanol in water.
The LabChip format eliminates the laborious manual staining and destaining
steps required with traditional SDS polyacrylamide gel electrophoresis.
The MALDIspot Kit includes the new ZipPlate(R) micro-SPE plate, the first 96-well sample preparation tool for destaining
, digesting, and concentrating proteins from either 1D or 2D gels in a single plate.
1% amino black) and destained in destaining
solution (20% methanol, 10% acetic acid, 70% double-distilled [H.
After electrophoresis, gels were removed from the glass plates and processed further for staining and destaining
in a washed plastic container.
, the change in bands' staining intensity was measured by scanning with gel documentation device (Gel Doc.
The dried gel is transferred to the staining module of the SPIFE system for automatically controlled washing, staining, destaining
, and drying.
Time consuming destaining
steps, required with conventional DNA detection methods, are not necessary with Vistra Green.