Worms were then destained
in 70% acidulated ethanol until a light pink color.
Then it was fixed, stained with Coomassie Brilliant Blue, destained
and kept in preservation solution.
The target band in SDS-PAGE was collected, destained
with Coomassie brilliant blue in 60% acetonitrile (ACN), reduced at 37[degrees]C by 1 M dithiothreitol (DTT) for 0.
The protein spots of interest were excised manually from the silver-stained gels, destained
, and subjected to tryptic digestion.
Next, the gel was destained
in a solution containing 250 mL methanol, 100 mL acetic acid, and 650 mL distilled water until protein fractions appeared clear.
Equal loading was determined using MEMCode total protein stain (Pierce) and then destained
before Western blotting.
The slices were cut into 1 mm cubes and destained
in acetonitrile/100 mmol/L N[H.
Prior to mounting, samples may be stained with toluidine blue and destained
in water for better contrast.
123% (w/v) coomassie blue R-250 in 50% (v/v) methanol and 10% (v/v) acetic acid for 30-45 min and destained
in 50% methanol and 10% acetic acid.
smears were rehydrated in cold Hank balanced salt solution for 10 minutes, the film collected by scraping with a scalpel blade, and the cellular residue transferred to a 1.
Gels were stained for 20 minutes using ethidium bromide, destained
in distilled water for 30 minutes, and then visualized and photographed in UV light using the Kodak 1D analysis software (Kodak 1D Image Analysis Software, 2000).
After electrophoresis, the gel was stained with ethidium bromide for 30 min, destained
in distilled water for 1 h, and DNA was visualized under UV light (Gel doc 1000, Bio-Rad Laboratories, Inc.