The delay would allow for females to depurate
pyrene for a longer period of time, leading to decreased pyrene in the oocytes, and less severe effects on the developing embryo, as compared to exposed females who immediately produced a brood after mating with solvent-control males.
The collectors were retrieved in July 2010, and crabs were held in the laboratory for 24 h to depurate
Upon arrival, the mussels were cleared of epibionts, placed in groups of 20 organisms in Nestier boxes suspended inside a 2-ton fiberglass tank filled with filtered seawater (10 [micro]m, 35 [+ or -1] 1 [per thousand], 26[degrees]C), and let to depurate
for 48-72 h before the inducement of spawning.
To conduct each experiment, 30 individual bivalve molluscs of one species were held for 48 h in FSW to depurate
previously-consumed algae and become acclimated to the experimental conditions (20[degrees]C in a temperature-controlled room with 24-h light).
ebena in each tank were given the opportunity to depurate
The clams were, therefore, allowed to depurate
over 72 h in the current study, after which all animals were collected for dissection, condition index, and inorganic content measurements.