References in periodicals archive ?
The deoxyuridine suppression test is a sensitive indicator of cobalamin and folate deficiency (42).
The PCR mixture consisted of SYBR[R] Green PCR Master Mix, which includes DNA polymerase, SYBR[R] Green I Dye, deoxynucleotide triphosphates including deoxyuridine triphosphate, PCR buffer, 10 pmol forward and reverse primers, and cDNA of samples in a total volume of 20 [micro]L.
Mutations are heritable changes in the DNA, can be either naturally occurring or man-made, and form the basis of much genetic variation [26], there are three main types of mutation: Substitution, Insertion and Deletion, mutagen is distinguished into chemical mutagen such as 5-bromo- deoxyuridine, colchicine and 2, 4 Dinitroaniline [28] and, physical mutagen such as UV, X-radiation and Gamma rays [27,28] and biological mutagen such as Helicobacter pylori bacterium [41].
In this reaction, one carbon group of the 5,10-MTHF is transferred to a molecule called deoxyuridine monophosphate (dUMP), resulting in the formation of deoxythymidine monophosphate (dTMP) and dihydrofolate (DHF).
A further method that can limit contamination is where the amplicon can be inactivated by incorporating deoxyuridine (dUTP) instead of dTTP in the PCR assay, so that any amplicons that may contaminate subsequent assays are cleaved by uracil-N-glycosylase (9).
The production of thymidine monophosphate (TMP) via the salvage route supplements de novo synthesis from intracellular deoxyuridine such that the rate of uptake of exogenous TdR or FLT is affected by the relative utilization of the salvage versus de novo pathways.
In addition, terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL)-positive cells are colocalized with FasL in MS lesions.
A more standard sterilization technique involves using deoxyuridine in place of thymidine in the reaction mixture.
To further reduce this risk, uracil-N-glycosylase and deoxyuridine triphosphate were used to prevent amplicon carryover (13).
Enhanced breadth of priming is achieved through the use of primers and probes containing 5-propynyl deoxycytidine and deoxyuridine nucleotides that offer increased affinity and base pairing selectivity (18,19).
Individual apoptotic cells were detected in 3 [micro]m sections by the method of transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL).