density-gradient centrifugation

density-gradient centrifugation

the separation of the components of an homogenate by a process of CENTRIFUGATION where each component is moved to a level equivalent to its density in the solute gradient used. The technique has a huge advantage over normal centrifugation, in that several components of different density can be separated in the same tube. Sucrose or caesium chloride is often used as the centrifugation medium. See DIFFERENTIAL CENTRIFUGATION, ULTRACENTRIFUGE, EQUILIBRIUM CENTRIFUGATION.
References in periodicals archive ?
X and Y spermatozoa can be separated using sorting methods such as flow cytometry, albumin sedimentation, and Percoll density-gradient centrifugation [13, 25].
Percoll density-gradient centrifugation method could be used to assess economic value and led to greater ease of use [27].
Sexing using Percoll density-gradient centrifugation method for 5 and 7 min was applied in this research.
Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation.
Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa.
Early attempts at purifying nucleic acids relied on density-gradient centrifugation, an expensive process that requires multiple steps and dangerous chemicals.
We isolated the lymphocyte cell fraction from cord blood samples collected at term and from fetal blood obtained from early pregnancies that were terminated (n = 12; refer to Table 1) by density-gradient centrifugation (Ficoll-Pague PLUS; Amersham Pharmacia Biotech AB).