denaturing gradient gel electrophoresis


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denaturing gradient gel electrophoresis (DGGE)

electrophoretic technique for separating DNA molecules on the basis of their sequence. A gradient of increasingly denaturing conditions, using a chemical denaturant such as formamide or urea, is applied across a gel and as double-stranded DNA molecules migrate through the gel, they denature into partially and then fully single-stranded DNA, with a consequent change in electrophoretic mobility, which dictates their final position in the gel.

The stability of the DNA helix to denaturation depends on the G+C content of the molecule. Thus DNA molecules of similar size, but different %G+C will denature and separate at different denaturant concentrations. The general principle of this technique can be applied to separate HETERODUPLEXES from HOMODUPLEXES based on the degree of COMPLEMENTARY BASE PAIRING between the two strands in each molecule.

A variation of this technique, temperature/ thermal gradient gel electrophoresis (TGGE), employs a temperature gradient, rather than chemical denaturants.

References in periodicals archive ?
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages.
Denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients.
Details of partial amoA sequences (150 amino acid) of denaturing gradient gel electrophoresis (DGGE) bands and their closest relatives DNA sequences can be accessed through DNA Data Base of Japan (DDBJ: www.
Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA--a new molecular approach to analyze the genetic diversity of mixed microbial communities, p.
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.
A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis.
AIM: To establish exclusive PCR (E-PCR) with denaturing gradient gel electrophoresis (DGGE) method as a novel approach by identifying cry4 subclass genes in mosquitocidal strains of B thuringiensis.
T-cell receptor [Gamma]chain gene analysis was performed by polymerase chain reaction (PCR) and analyzed on denaturing gradient gel electrophoresis as previously described.
Genetic differences within the symbiont populations of California anemones have been uncovered by restriction length polymorphism (RFLP) analysis of the small subunit (SSU) and large subunit (LSU) ribosomal RNA genes, and by denaturing gradient gel electrophoresis (DGGE) of the internal transcribed spacer region 2 (ITS 2).
This study also demonstrates the suitability of the chloroplast genome in addressing population-level questions, and the usefulness of denaturing gradient gel electrophoresis for assessing fine scale variation among and within populations.