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denature
(redirected from denaturing)

   Also found in: Dictionary/thesaurus, Legal, Encyclopedia, Wikipedia, Hutchinson 0.02 sec.
de·na·ture (d-nchr)
v.
1. To change the nature or natural qualities of.
2. To render unfit to eat or drink without destroying usefulness in other applications, especially adding methyl alcohol to ethyl alcohol.
3. To alter the chemical structure of a protein, as with heat, alkali, or acid, so that some of its original properties, especially its biological activity, are diminished or eliminated.

de·natur·ation n.

denature
to deprive a substance of its natural qualities, e.g. by heating a protein to destroy its specific biological activity.


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Real-time PCR conditions were a single hot-start cycle at 95[degrees]C for 30 s, followed by 55 cycles of denaturing at 94[degrees]C for 10 s, annealing at 58[degrees]C for 6 s (for Bartonella) or at 60[degrees]C for 6 sec (for the 4 Bartonella species), and final extension at 72[degrees]C for 10 s.
Denaturing polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA).
According to the government's announcement the test is a "more accurate genetic testing method, denaturing high performance liquid chromatography, or DHPLC screening.
 
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