denature

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denature

(dē-nā′chər)
tr.v. dena·tured, dena·turing, dena·tures
1. To change the nature or natural qualities of.
2. To render unfit to eat or drink without destroying usefulness in other applications, especially to add methanol to (ethyl alcohol).
3. Biochemistry
a. To cause the tertiary structure of (a protein) to unfold, as with heat, alkali, or acid, so that some of its original properties, especially its biological activity, are diminished or eliminated.
b. To cause the paired strands of (double-stranded DNA) to separate into individual single strands.

de·na′tur·ant n.
de·na′tur·a′tion n.

denature

(dē-nā′chŭr) [ de- + nature]
1. In chemistry, to change the qualities of a substance, esp. to make alcohol (ethyl alcohol) unfit to drink by adding an unpleasant ingredient, e.g. methanol.
2. In biochemistry, to make a change in conditions (temperature, addition of a substance) that causes an irreversible change in the structure of a protein, usually resulting in precipitation of the protein.
2. In genetics, to separate double-stranded DNA into two complementary strands, usually with heat.
denatured (′chŭrd), adjectivedenaturation (dē″nā″chŭ-rā′shŏn)

denature

to deprive a substance of its natural qualities, e.g. by heating a protein to destroy its specific biological activity.
References in periodicals archive ?
The heating treatment was done in order to mimic one denaturing step of the PCR cycle in absence of primers.
Denaturing high-performance liquid chromatography: a review.
The standardized cry4B product migrated to a position in the denaturing gradient gel equivalent to 54.
EndoVII digestion fragments were resolved on a 10% polyacrylamide denaturing MADGE gel containing 7 mol/L urea and 1x Tris-borate-EDTA buffer [90 mmol/L Tris-HCl (pH 8.
Detection of a molecular defect in 40 of 44 patients with haemophilia B by PCR and denaturing gradient gel electrophoresis.
Denaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations.
Detection of eleven mutations causing acute intermittent porphyria using denaturing gradient gel electrophoresis.
DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.
Heteroduplex molecules originate when a variation at the heterozygous state is present in a DNA fragment after denaturing and reannealing of the PCR product.
The Helix column offers professionals in biotechnology, large pharmaceutical companies, medical centers and universities a lower-cost alternative to gel electrophoresis that is ideal for denaturing HPLC and the analysis and purification of PCR (polymerase chain reaction) products and restrictions digests.
Simultaneous with the start up of this new SASOL production unit and as SASOL'S solvent distribution partner in North America, JLM will bring on line a state of the art computerized blending and denaturing facility dedicated to serving the North American ethanol market.
Some of these practices expose the protein to denaturing conditions such as heat or air, or leave residual product contamination which necessitates further processing.