denature

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denature

(dē-nā′chər)
tr.v. dena·tured, dena·turing, dena·tures
1. To change the nature or natural qualities of.
2. To render unfit to eat or drink without destroying usefulness in other applications, especially to add methanol to (ethyl alcohol).
3. Biochemistry
a. To cause the tertiary structure of (a protein) to unfold, as with heat, alkali, or acid, so that some of its original properties, especially its biological activity, are diminished or eliminated.
b. To cause the paired strands of (double-stranded DNA) to separate into individual single strands.

de·na′tur·ant n.
de·na′tur·a′tion n.

denature

(dē-nā′chŭr) [ de- + nature]
1. In chemistry, to change the qualities of a substance, esp. to make alcohol (ethyl alcohol) unfit to drink by adding an unpleasant ingredient, e.g. methanol.
2. In biochemistry, to make a change in conditions (temperature, addition of a substance) that causes an irreversible change in the structure of a protein, usually resulting in precipitation of the protein.
2. In genetics, to separate double-stranded DNA into two complementary strands, usually with heat.
denatured (′chŭrd), adjectivedenaturation (dē″nā″chŭ-rā′shŏn)

denature

to deprive a substance of its natural qualities, e.g. by heating a protein to destroy its specific biological activity.
References in periodicals archive ?
We demonstrated the formation of HDs using a simple system on denaturing gradient (DG) gel.
The denaturing approach is not completely distinct from earlier work, because it is based on that work.
Denaturing high-performance liquid chromatography: a review.
This system compares very favorably with denaturing gels and silver staining techniques that are widely used in many laboratories for genotyping with microsatellite markers.
This map predicts a location where DNA fragments will be denatured and stopped to form a band in a denaturing gradient gel (Lerman and Silverstein 1987).
Subsequently, 330 amplicons were screened blind with denaturing MADGE (below) as the analytical platform.
The percentage of substrate converted to product was determined after denaturing polyacrylamide gel electrophoresis using standard phosphorimager analysis as above.
The use of biocompatible options brings a streamlined approach to denaturing DNA and semi-preparative proteomics separations.
Denaturing gradient gel electrophoresis (3, 4) and direct gene sequencing (5-8) have been used for the direct identification of FIX mutations.
In addition, the current process of making gelatin by denaturing collagen (a protein extracted from the skin, bones, and tissues of animal carcasses) yields a highly variable material that is not easily traced to its source once incorporated into consumer products.
describe their MDE gel method in sufficient detail, the method is neither applicable to most clinical laboratories (which is not the intended audience), nor is it the method of choice for current gene scanning research efforts compared with denaturing HPLC (DHPLC), denaturing gradient gel electrophoresis (DGGE), or temperature gradient capillary electrophoresis (TGCE).
As such therapeutic proteins and enzymes retain structural and biological integrity and products are left without traces of denaturing solvents or potentially mutagenic chemicals.