were reported to possess dehalogenase
activity, and although it has not been demonstrated in isolates derived from the mammalian gut, they may contribute to the metabolism of TCDF and other halogenated compounds (Xun et al.
Quantitative PCR targeting 16s rRNA and reductive dehalogenase
genes simultaneously monitors multiple dehalococcoides strains.
In all of biology there is only one enzyme known to be able to break down carbon-fluoride bonds, fluoroacetate dehalogenase
Enzymes, like dehalogenase
, nitroreductase, peroxidase, laccase and nitrilase, have been discovered in plant sediments and soils.
Such a reductive dehalogenation is known to be catalyzed by the TCHQ dehalogenase
isolated from Sphingobium chlorophenolicum.
Substrate bacteria containing the TCE (trichloroethylene) reductive dehalogenase
enzyme can metabolize TCE in groundwater through an anaerobic process called reductive declorination (halorespiration).
The investigators manipulated the temperature and acidity of solutions containing crystals of haloalkane dehalogenase
from a nitrogen-fixing bacterium, Xanthobacter autotrophicus.
The HaloTag technology is based on a unique protein tag which has been engineered from a bacterial dehalogenase
to covalently attach to a set of synthetic ligands, such as fluorescent dyes, for cellular imaging applications or to resin and magnetic beads for protein interaction analysis applications.
In plants, GSTs can compose up to 2% of soluble proteins (Scalla & Roulet, 2002) and seven distinct classes are presently recognized, those are namely; Phi, Tau, Lambda, Dehydroascorbate reductases (DHARs), Theta, Zeta, and Tetrachlorohydroquinone dehalogenase
(TCHQD), with the exception of functional characterization reports on TCHQD.
Initial characterization of a reductive dehalogenase
from Desulfitobacterium chlororespirans Co23.
The present volume considers such topics as flavoenzymes involved in non-redox reactions, mechanisms of bacterial luciferase and related flavin reductases, choline oxidase and related systems, toward understanding the mechanism of oxygen activation by flavoprotein oxidases, glutamate synthase, and flavoprotein dehalogenases
Organophosphorus hydrolases, carbamate hydrolases, haloalkane dehalogenases
, and serine proteases, for example, have been shown to degrade various chemical warfare agents, pesticides and harmful microbes.