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The TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay was used to assess cardiomyocyte apoptosis, according to the instructions in the reagent kits (Roche Molecular Biochemicals).
As formulated into most premade reaction kits, application of ung contamination control therefore consists of nothing more than adding the ung to dUTP containing master mix (if not already present), and adding a few-minute PCR reaction preincubation step at moderate temperatures (usually 37[degrees]-45[degrees]C).
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue straining, chromomycin A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.
TDT is used to transfer the dUTP labeled with fluorescent marker (e.
Fluorescent terminal deoxynucleotide transferasemediated dUTP nick-end labelling (TUNEL) was performed on 5 Am-thick transverse sections of sample A.
On the basis of the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, it is reported that 50-80% of liver endothelial cells and hepatocytes die through apoptosis during the first 3-6 h of reperfusion [5].
TUNEL (terminal dUTP nick-end labeling) assays were performed at baseline and at week 4 to evaluate the percentage of sperm DNA fragmentation.
However this is of limited use unless the assay is established with dUTP i.
The agreement covers the rights for the use of dUTP (deoxyuridine triphosphate) in PCR-based (polymerase chain reaction) methods to prevent sample contamination by amplification products in aerosols.
Cell death was detected using the Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay at various capsaicin concentrations and exposure times.
For hematoxylin and eosin (H&E) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), eyes were immersion-fixed overnight in 4% paraformaldehyde in 0.
4 years) was grafted to beige SCID mice, and epidermal thickness, proliferation (Ki-67 expression), apoptosis (TUNEL [Tdt-mediated dUTP nick end labeling] reaction below granular layer), and expression of Fas and FasL were determined by histology and immunochemical staining.