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2] 200 [micro]M of dNTP containing each of dATP, dCTP, dGTP, and dTTP, 1 U of Taq DNA Polymerase, the PCR primers and 3 ([micro]of 1:10 diluted first reaction DNA.
The DTTP initiative is designed to improve readiness and the overall command and control process while providing an e-learning technology foundation that federal and state agencies, academia, business and industry can leverage," said Susan Dix, chief technology officer, DTTP initiative.
2]; Boehringer Mannheim GmbH), 100 [micro]M GeneAmp dNTP mixture with dTTP (Applied Biosystems, Branchburg, NJ, USA), 200 nM forward primer NVG1f1b (5'-CGY TGG ATG CGN TTC CAT GA-3') (24), 200 nM reverse primer G1SKR (5'-CCA ACC CAR CCA TTR TAC A-3') (25), and 5 [micro]L template DNA.
2], dNTPs (dATP, dGTP, dCTP, dTTP, 200 [micro]M each), and 5% DMSO.
A 10-[micro]L mix containing 4 [micro]L reverse transcription buffer (Invitrogen, Cergy Pontoise, France), 1 [micro],L dNTP (dATP, dTTP, dCTP, and dGTP, 25 mM each; Pharmacia, Saclay, France), 2 [micro]L 0.
In submitting her nomination, GODORT's DTTP award nominating committee noted, "her leadership, collegiality, and ability to articulate issues have contributed greatly to the cohesion and success of our organization.
18]-primed polymerization using SuperScript II reverse transcriptase (Invitrogen) in a total volume of 30 [micro]l including 25 mM each of dATP, dTTP, and dGTP, 10 mM of dCTP, and 3 mM of Cy3 or Cy5 labeled dCTP (NEN Life Science Product Inc.
2], 5 [micro]mol/L of dATP, dGTP, dTTP, and biotin-l4-dCTP (Invitrogen), 1 x reaction buffer, and 1.
Y02028; Gibco/ Invitrogen); 100 [micro]L MgCl2 (50 mM); 20 [micro]L each of dATP, dCTP, dGTP, dTTP (all 100 mM); 19.
2] and dNTPs (dATP, dGTP, dCTP and dTTP, 200 [micro]M each), and 5% DMSO.
Total RNA was first mixed with 2 [micro]L gene-specific mixture containing 55 primers (each antisense primer at 1 [micro]mol/L) and 2 [micro]L of 10 mmol/L dNTP Mix (10 mmol/L each dATP, dGTP, dCTP, dTTP at pH 7).
RNA (300-400 ng) was annealed with 500 ng random hexamer oligonudeotide (Amersham Biosciences), and cDNA probes were prepared using M-MLV reverse transcriptase as described by the manufacturer (Invitrogen) with the following modifications: the dNTP mix consisted of 500 [micro]mol dGTP, dTTP, and dCTP, and 4 [micro]mol dATP, and 50[micro]Ci [[alpha]-[sup.
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