dNTP


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dNTP

Deoxyribonucleotide triphosphate. A generic term referring to the four deoxyribonucleotides: dATP, dCTP, dGTP and dTTP.
References in periodicals archive ?
EGCG, EA, or RA treatment for 24 h each caused a significant imbalance of dNTPs in HL-60 cells, which was reminiscent of the imbalances caused by the RR inhibitors hydroxyurea and gemcitabine (Hakansson et al.
Extension reactions were performed as described above, except that unmodified dNTPs were used with the hot start polymerases and all 4 heat-activated dNTPs were used with Klentaq I.
Pyrosequencing, with its low coefficient of variation, is inherently more quantitative: Different dNTPs generate similar peak heights following single incorporation events, and the peak height generated from incorporating 3 dNTPs is 3 times the signal from incorporating one of the same dNTPs.
It then determines the response as various dNTPs are dispensed and maintains the position of the polymerase on each respective strand of DNA.
dNTPs in the environment diffuse into the polymerase active site.
The CleanAmp(TM) Primers will solve specific assay issues whereas the modified dNTPs will be a more general solution.
The reaction was performed in a total volume of 50 [micro]L containing 2 [micro]L RNA extract, 10 [micro]L 5x QIAGEN OneStep RT-PCR buffer, 2 [micro]L dNTPs mix (final concentration of 400 [micro]mol/L of each dNTP), 1.
2], 3 U of AmpliTaq Gold DNA polymerase (Applied Biosystems), 200 nM each primer, 1 mM dNTPs (Amersham Pharmacia Biotech), and 50 ng of the genomic DNA.
Individual dNTPs [TTP, dGTP, dCTP, or deoxyadenosine a-thio-triphosphate (dATPaS)] were sequentially added to the reactions after each elongation reaction step.
The DNA polymerase in a few tissues can catalyze the synthesis of DNA using only a primer and four dNTPs without a template (27), which is accompanied by release of [PP.